Extraction and Partial Characterization of Deacetylated Chitin from Cuttlefish Sepia kobiensis and their Free Radical Inhibition Efficacy

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DOI: 10.21522/TIJPH.2013.13.01.Art088

Authors : Pasiyappazham Ramasamy, Aarthi Lakshmanan, Yagniyasree Manogaran

Abstract:

This research aimed to extract and describe a functional polymeric compound from Sepia kobiensis cuttlebone and investigate its potential to impede the oxidation process. Cuttlebone waste is readily available and regularly discarded, making it an ideal resource for extracting useful bioactive compounds. The isolation technique created a pure polymeric substance by solvent extraction, precipitation, and purification. Several analytical methods, such as Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM), were utilized to describe the isolated polymeric material. The study found that the separated material has a specific polymeric structure with functional groups linked to antioxidant action. Furthermore, the study evaluated the bioactivity of the isolated substance by measuring its ability to suppress oxidation processes in a model system. The material demonstrated high antioxidant activity by preventing reactive oxygen species (ROS) formation and scavenging free radicals. This action was attributed to the presence of antioxidant moieties within the structure as well as its polymeric nature. Finally, a bioactive polymeric substance with significant antioxidant properties was successfully isolated and characterized from the cuttlebone of Sepia kobiensis. The findings demonstrate how cuttlebone debris may be a valuable source of bioactive compounds and aid in the development of naturally occurring antioxidants for application in the culinary, cosmetic, and pharmaceutical industries. Further research is required to properly investigate its bioactivities and understand the underlying mechanisms of action.


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