Green Synthesis of Selenium Nanoparticles using Vaccinium Subg. Oxycoccus for Antioxidant, Anti-Inflammatory, and Cytotoxic Effect
Abstract:
This
study elucidates the synthesis and multifaceted applications of selenium
nanoparticles (SeNPs) utilizing Vaccinium subg. Oxycoccus extract as a green
and sustainable methodology. The UV-visible spectroscopy analysis demonstrated
the successful synthesis of SeNPs, characterized by a distinct absorption peak
at 380 nm. The antioxidant activities of Vaccinium subg. Oxycoccus-mediated
SeNPs were systematically evaluated through three assays. The DPPH assay
revealed concentration-dependent radical scavenging activities, surpassing the
standard (ascorbic acid) at higher concentrations. Additionally, the Hydrogen
Peroxide assay showcased commendable antioxidant properties, while the FRAP
assay indicated a concentration-dependent capacity to reduce ferric ions,
suggesting potential in counteracting oxidative stress. Inhibition of protein
denaturation was examined using the BSA assay, revealing a significant
inhibitory effect of Vaccinium subg. Oxycoccus-mediated SeNPs that increased
with concentration. Denaturation studies, employing the Egg Albumin
Denaturation Assay, displayed a concentration-dependent rise in denaturation
percentages, either matching or exceeding the standard's anti-inflammatory
activity. The Membrane Stabilization Assay illustrated the
concentration-dependent enhancement of membrane stability by SeNPs, exhibiting
efficacy comparable to or exceeding the standard. Cytotoxicity assessment
through the Brine Shrimp lethality assay demonstrated a concentration-dependent
decline in brine shrimp nauplii viability, suggesting a potential cytotoxic
impact of Vaccinium subg. Oxycoccus-mediated SeNPs. These findings collectively
underscore the diverse applications of SeNPs synthesized with Vaccinium subg.
Oxycoccus extract, ranging from antioxidant activities and membrane
stabilization to potential cytotoxic effects, lays the groundwork for their
versatile application in biological and medicinal contexts.
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