Abstract:

Wound healing poses a critical clinical challenge, especially in chronic wounds where conventional treatments often fail. This study investigates the wound healing potential of β-Chitosan-copper oxide nanoparticles (β-Ch-CuO-NPs) at 10 μg/mL and 20 μg/mL using a zebrafish (Danio rerio) model. Zebrafish, renowned for their regenerative ability and transparent tissues, facilitated real-time observation of healing. The β-Ch-CuO-NP-treated groups exhibited enhanced wound closure, with the 20 μg/mL concentration outperforming the 10 μg/mL and control groups. These findings highlight that β-Ch-CuO-NPs can be used as a therapeutic remedy for wound healing, encouraging further translational and clinical research. To evaluate the wound healing efficacy of β-Chitosan-copper oxide nanoparticles (β-Ch-CuO-NPs) in zebrafish (Danio rerio). Chronic wounds pose a significant clinical challenge due to delayed healing and persistent inflammation. β-Ch-CuO-NPs have emerged as promising agents as they are much more biocompatible with properties such as antimicrobicity and the ability to modulate tissue repair mechanisms. Zebrafish were divided into control and treated groups receiving β-Ch-CuO-NPs at 10 μg/mL and 20 μg/mL. Wound healing was assessed through histological and molecular analyses over 14 days. The successful synthesis of β-Ch-CuO-NPs was confirmed by the characterization studies done, revealing a crystalline structure corresponding to copper oxide. Microscopic analysis of the zebrafish model revealed that the nanoparticles lead to a significant amount of wound closure when compared to the control. The 20 μg/mL group demonstrated superior wound closure, faster re-epithelialization, increased fibroblast proliferation, collagen deposition and reduced inflammation compared to other groups. β-Ch-CuO-NPs effectively promote wound healing by enhancing and accelerating wound closure, and tissue regeneration, indicating potential for clinical applications.

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