Abstract:

The glycocalyx or biofilm is essential for microbial survival. But dental biofilms cause cavities in teeth. The current study planned to incorporate silver nanoparticles and sodium fluoride in resin-modified glass-ionomer cement to test anti-biofilm efficacy. Silver nanoparticles (NAg) were chemically reduced by sodium borohydride. At 0.15% mass fraction, NAg was added in commercial resin-modified glass-ionomer cement (GC) and in this blend, 4 mass fractions (5%, 10%, 20%, and 30%) of NaF were mixed. The metabolic activity of biofilm, which shows the metabolic activity was decreased by NaF (P > .05). The biofilm CFU count shows the CFU counts of GC+NAg+20%NaF were decreased to 106 CFU/disk (P<.05). The cell viabilities of GC+NAg+20%NaF were 79.7%,75.6%,73% for days 1, 4, and 7 respectively, which were lower than GC control (P < .05) but higher than GC+NAg (P < .05). The use of NAg reduced cell viability at all three-time points (day 1, day 4, and day 7), which was partially reversed by the addition of NaF.

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