Green synthesis and Characterization of Copper oxide nanoparticles using Bauhinia tomentosa leaf extract and evaluation of its antimicrobial activity against wound pathogens
Abstract:
The increasing problem of antibiotic resistance in the treatment of
bacterial diseases has made the development of novel antimicrobial drugs
crucial. In this study, we examine the environmentally friendly synthesis of
copper oxide nanoparticles (CuONPs) using Bauhinia tomentosa (BT) leaf extract.
The plant B. tomentosa, which has been used for centuries for its therapeutic
uses, offers an eco-friendly and sustainable way to produce nanoparticles.
Diverse techniques such as Transmission Electron Microscopy (TEM), UV-Vis
spectroscopy, Scanning Electron Microscopy (SEM), Fourier transform infrared
spectroscopy (FTIR), and Energy-Dispersive X-ray spectroscopy (EDX), were used
to characterize these BT-CuONPs. The synthesis of CuO NPs is confirmed through
UV-Vis spectroscopy, showing an absorption peak at 225nm. Morphological
analysis via SEM and TEM reveals the presence of spherical and irregularly
shaped nanoparticles, typically around 50 nm in size. FTIR analysis identifies
characteristic absorption bands by indication of fuctional groups from leaf
extract that may interact with nanoparticles. Additionally, EDX analysis
confirms the elemental composition, predominantly revealing peaks for copper
and oxygen, validation successful synthesis of BT-CuONPs. BT-CuONPs were
synthesized and evaluated for their antimicrobial effectiveness against wound
pathogens, including multiple drug resistant organisms. The findings showed a
notable antimicrobial efficacy with highest inhibition zone against P.
aeruginosa at 40mm, suggesting that BT-CuONPs had strong antimicrobial
potential. The promise of employing plant extracts more especially, B.
tomentosa as a green synthesis route for CuONPs and their use in the fight
against drug-resistant microbial infections is highlighted in this study. In
the continuous fight against drug resistance, these successful production and
characterization of nanoparticles highlight their potential as strong antimicrobial
agents.
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