Camptothecin Anti-cancer Activity Against Breast Cancer Cells (MDA MB-231) Targeting the Gene Expression of Wnt/Beta-catenin Pathway - An In silico and In vitro Approach
Abstract:
Camptothecin, a potent anti-cancer agent, exhibits significant activity against MDA-MB-231 breast cancer cells by targeting the gene expression of the Wnt/β-catenin pathway. This pathway is crucial in cancer progression and cell proliferation. Camptothecin's effect on this pathway is elucidated through various assays and docking techniques. The DPPH assay demonstrates camptothecin's antioxidant potential, indicating its ability to neutralize free radicals. Additionally, nitric oxide assays reveal a significant enhancement in antioxidant properties, further supporting its therapeutic potential. Gene expression analysis provides insights into the molecular mechanisms underlying camptothecin's anti-cancer effects. The expression levels of key components of the Wnt/β-catenin pathway, including Wnt, β-catenin, APC, GSK3β, LP5, and Axin, are significantly altered in MDA-MB-231 cells upon camptothecin treatment. These changes suggest a disruption in the signaling pathway, which is vital for cancer cell survival and proliferation. The MTT assay results highlight camptothecin's capacity to inhibit cell growth in a time-dependent manner, underscoring its efficacy in reducing cancer cell viability over prolonged exposure. Moreover, docking studies indicate a high binding affinity between camptothecin and the Wnt/β-catenin pathway components, reinforcing the compound's role in modulating this critical signaling axis. Overall, camptothecin's multi-faceted approach, encompassing antioxidant activity and targeted gene expression modulation, presents a compelling case for its use in breast cancer therapy. The comprehensive analysis of its effects on the Wnt/β-catenin pathway offers valuable insights into its mechanism of action and potential as a therapeutic agent against aggressive breast cancer types like MDA-MB-231 cells.References:
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