Exploring Galium Aparine as a Promising Natural Therapy for Oral Squamous Cell Carcinoma

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DOI: 10.21522/TIJPH.2013.SE.24.06.Art003

Authors : Vishnu Priya Veeraraghavan, Chandini Rajkumar, Saranya Ramsridhar, Arul Prakash Francis, Krithika Udayappan, Amrutha M

Abstract:

Oral Squamous Cell Carcinoma (OSCC) is a head and neck cancer that has a detrimental impact on patients' quality of life. Surgery, radiation, and chemotherapy are standard treatment procedures that often have adverse effects and are limited in effectiveness, necessitating the quest for other therapeutic techniques.Galium aparine, a phytochemical, is currently acknowledged as a potentially effective treatment for OSCC. According to the literature, Galium aparine has a wide range of chemical elements, including flavonoids like quercetin and rutin, iridoids like asperuloside and aucubin, and phenolic acids like chlorogenic and caffeic acids. These chemicals have antioxidant, anticancer, and immunomodulatory properties, which make Galium aparine a potential natural treatment for OSCC.Preliminary investigations demonstrate its ability to inhibit cancer cell development in vitro and in vivo by inducing apoptosis and/or regulating immune responses. Furthermore, G.aparine extracts have been employed as hepatoprotective agents in a variety of malignancies, including breast carcinoma and melanoma.As a result of Galium aparineextracts’s proven antioxidant and anti-cancer properties in cancerous tissues elsewhere in the body, we have finally concluded that it might possess similar properties in OSCC. To fully understand its modes of action and possible impact on improving outcomes for patients with oral squamous cell carcinoma, more investigation and clinical trials are needed.This paper provides an overview of Galium aparine's biological activities, composition, and role as a powerful anti-cancer agent, as well as potential future research directions.

References:

[1].   Meier, J. K., Schuderer, J. G., Zeman, F., Klingelhöffer, C., Hullmann, M., Spanier, G., & Ettl, T., 2019, Health-related quality of life: a retrospective study on local vs. microvascular reconstruction in patients with oral cancer. BMC oral health, 19, 1-8.

[2].   Bagan, J., Sarrion, G. and Jimenez, Y., 2010, Oral cancer: clinical features. Oral oncology, 46(6), pp.414-417.

[3].   Warnakulasuriya, S., 2020, Oral potentially malignant disorders: A comprehensive review on clinical aspects and management. Oral Oncology, 102, p.104550.

[4].   Tarakji, B., 2022, Dentists’ perception of oral potentially malignant disorders. International Dental Journal, 72(3), pp.414-419.

[5].   Kerr, A.R. and Lodi, G., 2021, Management of oral potentially malignant disorders. Oral Diseases, 27(8), pp.2008-2025.

[6].   Selvaraj FM, Pillai VR, Joseph AP, Ramani P, Pazhani J, Mony V. Assessment of Tumor Budding in Different Grades of Oral Squamous Cell Carcinoma. Journal of Orofacial Sciences. 2023 Jul 1;15(2):160-6.

[7].   Tuorkey, M.J., 2015, Cancer therapy with phytochemicals: present and future perspectives. Biomedical and Environmental Sciences, 28(11), pp.808-819.

[8].   Orhan, N., Orhan, D.D., Aslan, M., Şüküroğlu, M. and Orhan, I.E., 2012, UPLC–TOF-MS analysis of Galium spurium towards its neuroprotective and anticonvulsant activities. Journal of ethnopharmacology, 141(1), pp.220-227.

[9].   Bokhari, J., Khan, M.R., Shabbir, M., Rashid, U., Jan, S. and Zai, J.A., 2013, Evaluation of diverse antioxidant activities of Galium aparine. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 102, pp.24-29.

[10].  Deliorman, D.İ.D.E.M., Calis, I. and Ergun, F., 2001, Iridoids from Galium aparine. Pharmaceutical Biology, 39(3), pp.234-235.

[11].  Simon, D., 2018, Recent advances in clinical allergy and immunology. International archives of allergy and immunology, 177(4), pp.324-333.

[12].  Vorob'ev, A.A., 2002, Principles of classification and the strategy of immunomodulators used in medicine. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii, (4), pp.93-98.

[13].  Vidalain, J.P., Onimus, M. and Michel, C.R., 1975, Long-term results of sub-astragal and mediotarsal arthrodesis. Revue de chirurgie orthopedique et reparatrice de l'appareil moteur, 61, pp.301-306.

[14].  Sun, H. X., Xie, Y. and Ye, Y. P., 2009, Advances in saponin-based adjuvants. Vaccine, 27(12), pp.1787-1796.

[15].  Mocan, A., Crișan, G., Vlase, L., Crișan, O., Vodnar, D.C., Raita, O., Gheldiu, A.M., Toiu, A., Oprean, R. and Tilea, I., 2014, Comparative studies on polyphenolic composition, antioxidant and antimicrobial activities of Schisandra chinensis leaves and fruits. Molecules, 19(9), pp.15162-15179.

[16].  Taylor, K., 1999, Galium aparine L. Journal of Ecology, 87(4), pp.713-730.

[17].  Mitova, M.I., Anchev, M.E., Handjieva, N.V. and Popov, S.S., 2002, Iridoid patterns in Galium L. and some phylogenetic considerations. Zeitschrift für Naturforschung C, 57(3-4), pp.226-234.

[18].  Ilina, T., Kashpur, N., Granica, S., Bazylko, A., Shinkovenko, I., Kovalyova, A., Goryacha, O. and Koshovyi, O., 2019, Phytochemical profiles and in vitro immunomodulatory activity of ethanolic extracts from Galium aparine L. Plants, 8(12), p.541.

[19].  Al-Snafi, A.E., 2022, Constituents, nutritional and pharmacological importance of Prunus persica-A review. World Journal of Advanced Pharmaceutical and Medical Research, 3(1), pp.019-029.

[20].  Kanso, M.A., Hijazi, M.A., El-Lakany, A. and Aboul-Ela, M., 2024, Review on phytochemical constituents and pharmacological activities of genus Galium. Journal of Applied Pharmaceutical Science.

[21].  Kuhtinskaja, M., Bragina, O., Kulp, M. and Vaher, M., 2020, Anticancer effect of the iridoid glycoside fraction from Dipsacus fullonum L. leaves. Natural Product Communications, 15(9), p.1934578X20951417.

[22].  Saar-Reismaa, P., Koel, M., Tarto, R. and Vaher, M., 2022, Extraction of bioactive compounds from Dipsacus fullonumleave using deep eutectic solvents. Journal of Chromatography A, 1677, p.463330.

[23].  Vorob'ev, A.A., 2002, Principles of classification and the strategy of immunomodulators used in medicine. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii, (4), pp.93-98.

[24].  Dai, J. and Mumper, R.J., 2010, Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), pp.7313-7352.

[25].  Ilina, T., Skowrońska, W., Kashpur, N., Granica, S., Bazylko, A., Kovalyova, A., Goryacha, O. and Koshovyi, O., 2020, Immunomodulatory activity and phytochemical profile of infusions from Cleavers herb. Molecules, 25(16), p.3721.

[26].  Bradic, J., Petkovic, A. and Tomovic, M., 2021, Phytochemical and Pharmacological properties of some species of the genus. Experimental and Applied Biomedical Research (EABR), 22(3), pp.187-193.

[27].  Arts, I.C. and Hollman, P.C., 2005, Polyphenols and disease risk in epidemiologic studies. The American journal of clinical nutrition, 81(1), pp.317S-325S.

[28].  Manach, C., Scalbert, A., Morand, C., Rémésy, C. and Jiménez, L., 2004, Polyphenols: food sources and bioavailability. The American journal of clinical nutrition, 79(5), pp.727-747.

[29].  Wang, C., Gong, X., Bo, A., Zhang, L., Zhang, M., Zang, E., Zhang, C. and Li, M., 2020, Iridoids: research advances in their phytochemistry, biological activities, and pharmacokinetics. Molecules, 25(2), p.287.

[30].  Liu, S., Khan, A.R., Yang, X., Dong, B., Ji, J. and Zhai, G., 2021, The reversal of chemotherapy-induced multidrug resistance by nanomedicine for cancer therapy. Journal of Controlled Release, 335, pp.1-20.

[31].  Naeem, A., Hu, P., Yang, M., Zhang, J., Liu, Y., Zhu, W. and Zheng, Q., 2022, Natural products as anticancer agents: current status and future perspectives. Molecules, 27(23), p.8367.

[32].  Harsha, C., Banik, K., Ang, H.L., Girisa, S., Vikkurthi, R., Parama, D., Rana, V., Shabnam, B., Khatoon, E., Kumar, A.P. and Kunnumakkara, A.B., 2020, Targeting AKT/mTOR in oral cancer: mechanisms and advances in clinical trials. International journal of molecular sciences, 21(9), p.3285.

[33].  Christianto, S., Li, K.Y., Huang, T.H. and Su, Y.X., 2022, The Prognostic Value of Human Papilloma Virus Infection in Oral Cavity Squamous Cell Carcinoma: A Meta‐Analysis. The Laryngoscope, 132(9), pp.1760-1770.

[34].  Cavalcante, G.C., Schaan, A.P., Cabral, G.F., Santana-da-Silva, M.N., Pinto, P., Vidal, A.F. and Ribeiro-dos-Santos, Â., 2019, A cell’s fate: an overview of the molecular biology and genetics of apoptosis. International journal of molecular sciences, 20(17), p.4133.

[35].  Schmidt, M., Polednik, C., Roller, J. and Hagen, R., 2014, Galium verum aqueous extract strongly inhibits the motility of head and neck cancer cell lines and protects mucosal keratinocytes against toxic DNA damage. Oncology Reports, 32(3), pp.1296-1302.

[36].  Zhang, J., Zheng, G., Zhou, L., Li, P., Yun, M., Shi, Q., Wang, T. and Wu, X., 2018, Notch signalling induces epithelial-mesenchymal transition to promote metastasis in oral squamous cell carcinoma. International journal of molecular medicine, 42(4), pp.2276-2284.

[37].  Zong, W.X., Rabinowitz, J.D. and White, E., 2016, Mitochondria and cancer. Molecular cell, 61(5), pp.667-676.

[38].  Carr, C., Ng, J. and Wigmore, T., 2008, The side effects of chemotherapeutic agents. Current Anaesthesia & Critical Care, 19(2), pp.70-79.

[39].  Kelland, L., 2007, The resurgence of platinum-based cancer chemotherapy. Nature Reviews Cancer, 7(8), pp.573-584.

[40].  Kayl, A.E. and Meyers, C.A., 2006, Side-effects of chemotherapy and quality of life in ovarian and breast cancer patients. Current opinion in obstetrics and gynaecology, 18(1), pp.24-28.

[41].  Laanet, P.R., Saar-Reismaa, P., Jõul, P., Bragina, O. and Vaher, M., 2023, Phytochemical screening and antioxidant activity of selected Estonian Galium species. Molecules, 28(6), p.2867.

[42].  Hamdi, O.H., Saadedin, S.M. and Al_Zaidi, I.H., 2021, Green biosynthesis of silver nanoparticles using gallium aparine green part extract and anti-skin cancer activity. Medico-legal Update, 21(2), pp.908-913.

[43].  Atmaca, H., Bozkurt, E., Cittan, M. and Tepe, H.D., 2016, Effects of Galium aparine extract on the cell viability, cell cycle and cell death in breast cancer cell lines. Journal of ethnopharmacology, 186, pp.305-310.

[44].  Sahin, B., Karabulut, S., Filiz, A.K., Özkaraca, M., Gezer, A., Akpulat, H.A. and Ataseven, H., 2022, Galium aparine L. protects against acetaminophen-induced hepatotoxicity in rats. Chemico-Biological Interactions, 366, p.110119.

[45].  Mazzio, E.A. and Soliman, K.F., 2010, In vitro screening of tumoricidal properties of international medicinal herbs: part II. Phytotherapy Research, 24(12), pp.1813-1824.

[46].  Corrigan, D., Timoney, R.F. and Donnelly, D.M., 1978, Iridoids and alkanes in twelve species of Galium and Asperula. Phytochemistry, 17(7), pp.1131-1133.

[47].  Ramasubramanian A, Arumugam P, Ramani P, Kannan BC, Murugan MS. Identification of Novel Cytochrome C1 (CYC1) Gene Expression in Oral Squamous Cell Carcinoma- An Evaluative Study. Ann Maxillofac Surg. 2022 Jul-Dec;12(2):144-150. doi: 10.4103/ams.ams_26_22. Epub 2022 Aug 24. PMID: 36874769; PMCID: PMC9976869.

[48].  Renu, K., 2024. A molecular viewpoint of the intricate relationships among HNSCC, HPV infections, and the oral microbiota dysbiosis. Journal of Stomatology, Oral and Maxillofacial Surgery, p.102134.

[49].  Subramanian, A.K. and Balakrishnan, N., Evaluation of Biological Response Elicited by Two Novel Tooth Cream Formulations of Cocos nucifera-Cell Line Studies and MTT Assay on Human Gingival Fibroblast.

[50].  Kaarthikeyan, G., Jayakumar, N.D. and Sivakumar, D., 2019. Comparative Evaluation of Bone Formation between PRF and Blood Clot Alone as the Sole Sinus-Filling Material in Maxillary Sinus Augmentation with the Implant as a Tent Pole: A Randomized Split-Mouth Study. Journal of long-term effects of medical implants, 29(2).

[51].  Kavarthapu, A. and Malaiappan, S., 2019. Comparative evaluation of demineralized bone matrix and type II collagen membrane versus eggshell powder as a graft material and membrane in rat model. Indian Journal of Dental Research, 30(6), pp.877-880.

[52].  Manchery, N., John, J., Nagappan, N., Subbiah, G.K. and Premnath, P., 2019. Remineralization potential of dentifrice containing nanohydroxyapatite on artificial carious lesions of enamel: A comparative: in vitro: study. Dental research journal, 16(5), pp.310-317.