Inhibition of Biofilm Formation and Pyocyanin Production from Multidrug Resistance P. aeruginosa by Using Vitamin C, Salicylic Acid, and Multisera
Abstract:
Pseudomonas aeruginosa is a potent
nosocomial pathogen, causing several infections, mostly urinary tract
infections (UTIs). The present study is thus aimed to detect the susceptibility
pattern of Pseudomonas aeruginosa to antibiotics from urine specimens, and virulence
factor production such as (biofilm, and pyocyanin) which are regulated by
quorum sensing. The aims were extended to detect the Inhibition of Biofilm
formation and pyocyanin production by using vitamin C, salicylic acid and Multi
sera. A total of 245 samples were collected from the
patients. The samples were subjected to inoculation, isolation and
identification of Pseudomonas aeruginosa which were 8 (4%) from total isolates,
by standard microbiological procedures. Confirmation of isolates was done by polymerase chain
reaction targeting 16srRNA.
Antimicrobial sensitivity testing was done using the modified
Kirby-Bauer method of the disc diffusion test, with high
resistance rates against ceftriaxone (100%) while high sensitivity was to meropenem
(88%). Quorum
sensing Genes (lasl, lasR) were also detected in Multi-drug resistance and pan
resistance isolates. In general, all isolates were Biofilm formation and
pyocyanin production. The highest Inhibition of Biofilm was by using vitamin C
while the highest
Inhibition of pyocyanin was by using salicylic
acid.
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