Prevalence of Multi-Drug Resistant Bacterial Isolates in Healthcare Environments
Abstract:
To
evaluate and measure the frequency of multi-drug resistance bacterial isolates
in various healthcare environments. This study employed the disc diffusion
susceptibility test to assess the antibiotic resistance of Acinetobacter, E.
coli, Klebsiella sp., and Pseudomonas sp. to carbapenem, extended-spectrum
β-lactamase (ESBL), and colistin. In the present study, Acinetobacter was
generally found resistant to carbapenem at 72% and 71% at 75 µg/ml and minimum
for Carbapenem + Colistin at 11% at 150 µg/ml. E. coli counts of the
antibiotic-resistant carbapenem samples were 85% and 71% at 150 µg/ml and the
lowest in colistin 12% at 225 µg/ml. The Klebsiella isolates obtained were
highly sensitive to carbapenem (98%) at 150 µg/ml, followed by 75 µg/ml (96%)
and 225 µg/ml (92%). Low colistin resistance was also observed (7%). The
evaluation presented in Pseudomonas sp. of the antibiotic-resistant carbapenem
samples was high at 47 at 225 µg/ml, and minimum in ESBL + Colistin at 5% at
150 µg/ml. Klebsiella (92%), Acinetobacter (71%), E. coli (64%), and
Pseudomonas sp. (47%) were highly sensitive to 75 µg/ml carbapenem. The MICs of
carbapenem-resistant Acinetobacter were shown in the 6.45 (OD values) at 75
µg/mL. Carbapenem and ESBL antibiotic-resistant Klebsiella were found at OD
values of 9.14, 9.74, 9.61, and 3.45, 3.21 3.67, (OD values) at 75, 150, and
225 µg/mL, respectively, and the highest susceptibility was observed with
Carbapenem 9.74 at 150 µg/mL. The ternary colour frequency of the graph indicates
that Klebsiella sp. showed good susceptibility to carbapenem, carbapenem +
ESBL, carbapenem, and colistin. Carbapenem is a highly effective therapy
against infections caused by antibiotic-resistant Klebsiella sp.
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