Molecular Mechanisms to Identify Anticancer Activity of Tomentin in A549 Lung Adeno Carcinoma Cells: Role of p53/Caspase-Mediated Pathways
DOI: 10.21522/TIJPH.2013.SE.24.03.Art012
Authors : Vishnu Priya Veeraraghavan, Selvaraj Jayaraman, Jerine I
Abstract:
Cancer,
a multifaceted disease with increasing prevalence, remains a significant global
health concern. Lung cancer, in particular, presents a formidable challenge due
to its high mortality rates. Tomentin, a phytochemical extracted from
Sphaeralcea angustifolia, has garnered interest for its potential anti-cancer
properties. This study employs both in vitro and in silico methods to elucidate
tomentin's efficacy against lung cancer, focusing on the A549 cell line, a
model for lung adenocarcinoma. The study begins by exploring the cytotoxic
effects of tomentin on A549 cells through viability assays, apoptosis
induction, and molecular pathway modulation. Results indicate dose-dependent
inhibition of cell proliferation and activation of apoptotic pathways by tomentin
treatment. Further analysis reveals tomentin's ability to scavenge DPPH
radicals and inhibit protein denaturation, suggesting potent antioxidant and
anti-inflammatory properties. Moreover, mRNA expression analysis demonstrates
tomentin's regulatory effects on key genes involved in inflammation and
apoptosis. Molecular docking studies reveal strong binding affinity between
tomentin and critical proteins implicated in cancer progression, including
MCL1, p53, Bcl-2, and Caspases.
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