Studies on the Antioxidant Role of Β-Sitosterol on Neuronal Cell Line (IMR32) In Vitro: Role of NRF2-Keap Pathway
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DOI: 10.21522/TIJPH.2013.SE.24.03.Art006
Authors : Selvaraj Jayaraman, Vishnu Priya Veeraraghavan, Ponnulakshmi Rajagopal, Chella Perumal Palanisamy, Ramajayam Govindan, S. Prathiba, R. Jayasree, Mahesh Kumar
Abstract:
β-Sitosterol is a phytosterol which is universally present in most of the plant seeds and nuts. Its anti-inflammatory property has been studied in vitro in cell line studies and some in vivo studies have also been conducted but few studies have been done to establish its antioxidant property. In this study, we have attempted to explore the antioxidant property through the NRF2-KEAP pathway in the IMR 32 neuronal cell line. Initially, cell viability was checked for varying concentrations of sitosterol using an MTT assay. There was a decline in viability for increased concentration and time factors. Reactive oxygen species (ROS) assay revealed the tendency of sitosterol to inhibit lipid peroxides and hydrogen peroxides. An antioxidant activity assay depicted activating antioxidant enzymes, superoxide dismutase, and nonenzymatic antioxidants, such as reduced glutathione. In the gene expression analysis using Real Time-PCR NRF2 and KEAP expression was found to be observed maximum for concentration ranges of 10 µM concentration of β-Sitosterol. Current studies have substantiated the antioxidant property of β-Sitosterol through the NRF2- KEAP signalling pathway.References:
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