Abstract:

Glyphosate is used as an herbicide in agriculture. At sub-agriculture concentrations, glyphosate-based herbicide inhibits cell proliferation. Glyphosate is a chelating agent that interferes with the metabolic activities in plants thereby adversely affecting its metabolism. The study aimed to determine the glyphosate-induced detrimental changes in SREBP-1c and PPAR-γ mRNA expression in adipose tissue of adult male rats. Adult male Wistar albino rats were divided into 4 groups, each consisting of 6 animals. Group I served as normal control rats; Group II-IV consisted of rats exposed to glyphosate at different concentrations (50, 100, and 250 mg/kg body weight respectively) orally for 16 weeks. After 16 weeks of treatment, the animals were sacrificed, and adipose tissue was dissected out for the assessment of SREBP-1c and PPAR-γ mRNA by real-time PCR using gene-specific down-regulated primers. The results with the p<0.05 level were considered to be statistically significant. The results showed a significant dose-dependent increase (P <0.05) in the expression of SREBP-1c in all the glyphosate-exposed rats compared to control rats and PPAR-γ mRNA expression was found to be significantly reduced in a concentration-dependent manner (P<0.05) compared to normal control animals. The current findings for the first time report that glyphosate had detrimental changes in the expression of transcription factors such as SREBP-1c and PPAR-γ mRNA in adipose tissue and thereby glyphosate may lead to the development of type-2 diabetes or insulin resistance.

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