Abstract:

Diabetes is a chronic disease that occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin it produces. Type 2 diabetics are insulin resistant. Insulin acts on AKT signaling molecules which then act on GLUT 4 which increases glucose uptake and glucose in the case of diabetic patients this pathway is absent to indicate that the pathway piperine is used. This study aims to evaluate the effect of piperine on Ir/ IRS-1/AKT signalling molecules in high-fat diet and sucrose-fed type 2 diabetic rats. Healthy adult male albino rats of Wister strain weighing 180 to 200 g were used for the evaluation of the effect of piperine. Fasting blood glucose and serum insulin levels are decreased with piperine administration. From this study, it is proved that piperine activates the insulin signaling pathway thus proving the potential anti-diabetic role.

References:

[1] Qaid MM, Abdelrahman MM (2016) Role of insulin and other related hormones in energy metabolism—A review. Cogent Food & Agriculture 2:1267691

[2] Himsworth H, Kerr R (1939) Insulin-sensitive and insulin-insensitive types of diabetes mellitus. Clin Sci 4:119–152

[3] Rauscher FM, Sanders RA, Watkins JB 3rd (2000) Effects of piperine on antioxidant pathways in tissues from normal and streptozotocin-induced diabetic rats. J Biochem Mol Toxicol 14:329–334

[4] Sun X, Veeraraghavan VP, Surapaneni KM, Hussain S, Mathanmohun M, Alharbi SA, Aladresi AAM, Chinnathambi A (2021) Eugenol-piperine loaded polyhydroxy butyrate/polyethylene glycol nanocomposite-induced apoptosis and cell death in nasopharyngeal cancer (C666-1) cells through the inhibition of the PI3K/AKT/mTOR signaling pathway. J Biochem Mol Toxicol 35:e22700

[5] Prasad M, Jayaraman S, Natarajan SR, Veeraraghavan VP, Krishnamoorthy R, Gatasheh MK, Palanisamy CP, Elrobh M (2023) Piperine modulates IR/Akt/GLUT4 pathways to mitigate insulin resistance: Evidence from animal and computational studies. Int J Biol Macromol 253:127242

[6] Mitra S, Anand U, Jha NK, Shekhawat MS, Saha SC, Nongdam P, Rengasamy KRR, Proćków J, Dey A (2022) Anticancer Applications and Pharmacological Properties of Piperidine and Piperine: A Comprehensive Review on Molecular Mechanisms and Therapeutic Perspectives. Front Pharmacol. https://doi.org/10.3389/fphar.2021.772418

[7] Yang W, Chen Y-H, Liu H, Qu H-D (2015) Neuroprotective effects of piperine on the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Parkinson’s disease mouse model. Int J Mol Med 36:1369–1376

[8] Jayachandran P, Gayathri R, Jayaraman S, Vishnu Priya V, Kavitha S (2023) Antioxidative Stress Potential of Piperine in the Gastrocnemius Muscle of High Fat Diet and Sucrose Induced Type 2 Diabetic Rats. HIV AIDS 23:81–89

[9] Karthika, J., Rajeev, Chandran, K. (2019). Morphological Comparison of the Changes in the Gingiva of Albino Wistar Rats on Administering Tacrolimus and Sirolimus Separately: An Experimental Study. The journal of contemporary dental practice, doi: 10.5005/JP-JOURNALS-10024-2532.

[10] Histomorphometric effects of energy drink consumption on pancreatic tissue of albino wistar rats. The professional medical journal, (2023). doi: 10.29309/tpmj/2023.30.06.7399

[11] Deenadayalan A, Subramanian V, Paramasivan V, Veeraraghavan VP, Rengasamy G, Coiambatore Sadagopan J, Rajagopal P, Jayaraman S (2021) Stevioside Attenuates Insulin Resistance in Skeletal Muscle by Facilitating IR/IRS-1/Akt/GLUT 4 Signaling Pathways: An In Vivo and In Silico Approach. Molecules. https://doi.org/10.3390/molecules26247689

[12] Deshpande KC, Kulkarni MM, Rajput DV (2018) Evaluation of glutathione peroxidase in the blood and tumor tissue of oral squamous cell carcinoma patients. J Oral Maxillofac Pathol 22:447

[13] Ravikumar D, R M, Ningthoujam S, Robindro W, R G, Priya V V (2018) Genotypic characterization of Streptococcus mutans in child-mother pair-A PCR based study. J Oral Biol Craniofac Res 8:225–230

[14] Daniel P (2023) Effect of lupeol on insulin resistance in adipose tissue by modulating the expression of insulin and inflammatory signaling molecules in high-fat diet and sucrose-fed diabetic rats. Bioinformation. https://doi.org/10.6026/97320630019445

[15] Wang X, Zhang Y, Zhang L, Wang W, Che H, Zhang Y (2022) Piperine attenuates hepatic steatosis and insulin resistance in high-fat diet-induced obesity in Sprague-Dawley rats. Nutr Res 108:9–21

[16] Jiang S, Ma F, Lou J, Li J, Shang X, Li Y, Wu J, Xu S (2024) Naringenin reduces oxidative stress and necroptosis, apoptosis, and pyroptosis in random-pattern skin flaps by enhancing autophagy. Eur J Pharmacol 176455

[17] Longevity OMAC (2023) Retracted: Activation of PTEN/P13K/AKT Signaling Pathway by miRNA-124-3p-Loaded Nanoparticles to Regulate Oxidative Stress Attenuates Cardiomyocyte Regulation and Myocardial Injury. Oxid Med Cell Longev 2023:9768785

[18] Atal S, Agrawal RP, Vyas S, Phadnis P, Rai N (2012) Evaluation of the effect of piperine per se on blood glucose level in alloxan-induced diabetic mice. Acta Pol Pharm 69:965–969

[19] [No title]. https://www.pnrjournal.com/index.php/home/article/download/1227/1008. Accessed 21 Nov 2023

[20] Vishaka S, Sridevi G, Selvaraj J (2022) An in vitro analysis on the antioxidant and anti-diabetic properties of Kaempferia galanga rhizome using different solvent systems. J Adv Pharm Technol Res 13:S505

[21] Jwa H, Choi Y, Park U-H, Um S-J, Yoon SK, Park T (2012) Piperine, an LXRα antagonist, protects against hepatic steatosis and improves insulin signaling in mice fed a high-fat diet. Biochem Pharmacol 84:1501–1510.

[22] Yuan Y, Zhou J, Hu R, Zou L, Ji L, Jiang G (2021) Piperine protects against pancreatic β-cell dysfunction by alleviating macrophage inflammation in obese mice. Life Sci 274:119312.

[23] Ealla KKR, Veeraraghavan VP, Ravula NR, Durga CS, Ramani P, Sahu V, Poola PK, Patil S, Panta P (2022) Silk Hydrogel for Tissue Engineering: A Review. J Contemp Dent Pract 23:467–477

[24] Patil S, Sujatha G, Varadarajan S, Priya VV (2022) A bibliometric analysis of the published literature related to toothbrush as a source of DNA. World J Dent 13:S87–S95

[25] Ganesan A, Muthukrishnan A, Veeraraghavan V (2021) Effectiveness of Salivary Glucose in Diagnosing Gestational Diabetes Mellitus. Contemp Clin Dent 12:294–300

[26] Karthik EVG, Priya V (2021) Gayathri. R, Dhanraj Ganapathy. Health Benefits Of Annona Muricata-A Review. Int J Dentistry Oral Sci 8:2965–2967

[27] Priya DV, (2020) Knowledge and awareness on HIV/AIDS among college students in A university hospital setting. Int J Dent Oral Sci 1182–1186

[28] Ganapathy D, (2021) Awareness of hazards caused by long-term usage of polyethylene terephthalate (PET) bottles. Int J Dent Oral Sci 2976–2980