Abstract:

Phytochemicals are secondary metabolites which act as antioxidants and possess various protective role.Alpha Amylase and Alpha Glucosidase enzymes catalyse hydrolysis of starch to simple sugars. Abelmoschus esculentus is a flowering plant of the Malvaceae family and is valued for its green seed pods. It naturally possesses anti-diabetic property, acts as a starch blocker as it prevents the absorption of starch in the body. Abelmoschus esculentus was cut vertically to open and added to 50ml of water. Soaked overnight and the aqueous extract was decanted and used for the phytochemical screening test followed by the evaluation of antioxidant and antidiabetic potential. The data were analysed statistically by a one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test. Overnight soaked extract of Abelmoschus esculentus exhibited a significant antioxidant potential (IC50=280 µg/ml) and compared with standard vitamin C. Alpha amylase and alpha glucosidase inhibitory potential of Abelmoschus esculentus was analysed (IC50=380µg/ml and 320µg/ml respectively) and compared with standard acarbose. Though Abelmoschus esculentus is a common vegetable, its efficiency increases when soaking overnight.

References:

[1] Anjani PP, Damayanthi E, Rimbawan, Handharyani E (2018) Antidiabetic potential of purple okra (Abelmoschus esculentus L.) extract in streptozotocin-induced diabetic rats. IOP Conference Series: Earth and Environmental Science 196:012038

[2] Ibrahim MA, Koorbanally NA, Islam MS (2014) Antioxidative Activity and Inhibition of Key Enzymes Linked to Type-2 Diabetes (α-Glucosidase and α-Amylase) by Khaya Senegalensis. Acta Pharmaceutica 64:311–324.

[3] Assefa ST, Yang E-Y, Chae S-Y, Song M, Lee J, Cho M-C, Jang S (2019) Alpha Glucosidase Inhibitory Activities of Plants with Focus on Common Vegetables. Plants 9:2.

[4] Kazeem MI, Adamson JO, Ogunwande IA (2013) Modes of Inhibition ofα-Amylase andα-Glucosidase by Aqueous Extract of Morinda lucida Benth Leaf. BioMed Research International 2013:1–6.

[5] Ogundajo A, Kazeem M, Owoyele O, Ogunmoye A, Ogunwande I (2016) Inhibition of α-amylase and α-glucosidase by Acanthus montanus Leaf Extracts. British Journal of Pharmaceutical Research 9:1–8.

[6] Mahindrakar KV, Rathod VK (2020) Antidiabetic potential evaluation of aqueous extract of waste Syzygium cumini seed kernel’s by in vitro α-amylase and α-glucosidase inhibition. Preparative Biochemistry & Biotechnology 1–10.

[7] Oboh G (2013) Inhibition of -amylase and -glucosidase activities by ethanolic extract of Amaranthus cruentus leaf as affected by blanching. African Journal of Pharmacy and Pharmacology 7:1026–1032.

[8] Chipiti T, Ibrahim MA, Singh M, Islam MS (2015) In vitro α-amylase and α-glucosidase inhibitory effects and cytotoxic activity of Albizia antunesiana extracts. Pharmacogn Mag 11:S231–6.

[9] Wu F, Zhu J, Li G, Wang J, Veeraraghavan VP, Krishna Mohan S, Zhang Q (2019) Biologically synthesized green gold nanoparticles from Siberian ginseng induce growth-inhibitory effect on melanoma cells (B16). Artif Cells Nanomed Biotechnol 47:3297–3305.

[10] Chen F, Tang Y, Sun Y, Veeraraghavan VP, Mohan SK, Cui C (2019) 6-shogaol, a active constiuents of ginger prevents UVB radiation mediated inflammation and oxidative stress through modulating NrF2 signaling in human epidermal keratinocytes (HaCaT cells). J Photochem Photobiol B 197:111518.

[11] Li Z, Veeraraghavan VP, Mohan SK, et al (2020) Apoptotic induction and anti-metastatic activity of eugenol encapsulated chitosan nanopolymer on rat glioma C6 cells via alleviating the MMP signaling pathway. Journal of Photochemistry and Photobiology B: Biology 203:111773.

[12] Babu S, Jayaraman S (2020) An update on β-sitosterol: A potential herbal nutraceutical for diabetic management. Biomed Pharmacother 131:110702.

[13] Malaikolundhan H, Mookkan G, Krishnamoorthi G, Matheswaran N, Alsawalha M, Veeraraghavan VP, Krishna Mohan S, Di A (2020) Anticarcinogenic effect of gold nanoparticles synthesized from Albizia lebbeck on HCT-116 colon cancer cell lines. Artif Cells Nanomed Biotechnol 48:1206–1213.

[14] Han X, Jiang X, Guo L, Wang Y, Veeraraghavan VP, Krishna Mohan S, Wang Z, Cao D (2019) Anticarcinogenic potential of gold nanoparticles synthesized from Trichosanthes kirilowii in colon cancer cells through the induction of apoptotic pathway. Artif Cells Nanomed Biotechnol 47:3577–3584.

[15] Gothai S, Muniandy K, Gnanaraj C, et al (2018) Pharmacological insights into antioxidants against colorectal cancer: A detailed review of the possible mechanisms. Biomed Pharmacother 107:1514–1522.

[16] Hassan A, Akmal Z, Khan N (2020) The Phytochemical Screening and Antioxidants Potential of Schoenoplectus triqueter L. Palla. Journal of Chemistry 2020:1–8.

[17] Nishi Y, Hatano S, Aihara K, Kihara M (1989) [Significance of copper analysis in clinical tests]. Nihon Rinsho 48 Suppl:771–774.

[18] Ademiluyi AO, Oboh G, Agbebi OJ, Akinyemi AJ (2013) Anthocyanin - Rich Red Dye of Hibiscus Sabdariffa Calyx Modulates Cisplatin-induced Nephrotoxicity and Oxidative Stress in Rats. Int J Biomed Sci 9:243–248.

[19] Jepkorir M, Ambundo T, Ngule C, Ndungu J, Njuguna D, Mbugua R, Chepngetich J, Mwitari P (2018) Phytochemical Screening and in vitro Antiproliferative Activity of the Fruit of Annona muricata and Abelmoschus esculentus Pods against Selected Cancer Cell Lines. Journal of Complementary and Alternative Medical Research 5:1–11.

[20] Khan TA, Fariduddin Q, Yusuf M, Ahmad A (2015) Low-Temperature Triggered Varied Antioxidant Responses in Tomato. International Journal of Vegetable Science 21:329–343.

[21] Rashmi P, Scholar PD, P. G. Dept. of Dravyaguna Vigyan, National Institute of Ayurveda, Jaipur (2017) Anti diabetic potential of some selected traditionally used Medicinal Plants in Western Ghats of India w.s.r to Prameha. International Journal of Ayurvedic and Herbal Medicine. https://doi.org/10.18535/ijahm/v7i4.05

[22] Ponnulakshmi R, Shyamaladevi B, Vijayalakshmi P, Selvaraj J (2019) In silico and in vivo analysis to identify the antidiabetic activity of beta sitosterol in adipose tissue of high fat diet and sucrose induced type-2 diabetic experimental rats. Toxicol Mech Methods 29:276–290.

[23] Indumathi D, Jayashree S, Selvaraj J, Sathish S, Mayilvanan C, Akilavalli N, Balasubramanian K (2013) Effect of bisphenol-A on insulin signal transduction and glucose oxidation in skeletal muscle of adult male albino rat. Hum Exp Toxicol 32:960–971.

[24] Rajesh P, Sathish S, Srinivasan C, Selvaraj J, Balasubramanian K (2013) Phthalate is associated with insulin resistance in adipose tissue of male rat: role of antioxidant vitamins. J Cell Biochem 114:558–569.

[25] Srinivasan C, Khan AI, Balaji V, Selvaraj J, Balasubramanian K (2011) Diethyl hexyl phthalate-induced changes in insulin signaling molecules and the protective role of antioxidant vitamins in gastrocnemius muscle of adult male rat. Toxicology and Applied Pharmacology 257:155–164.

[26] Williams AA, Selvaraj J, Srinivasan C, Sathish S, Rajesh P, Balaji V, Arunakaran J, Balasubramanian K (2013) Protective role of lycopene against Aroclor 1254-induced changes on GLUT4 in the skeletal muscles of adult male rat. Drug Chem Toxicol 36:320–328.

[27] Satyanarayana K, Sravanthi K, Shaker IA, Ponnulakshmi R, Selvaraj J (2015) Role of chrysin on expression of insulin signaling molecules. J Ayurveda Integr Med 6:248–258.

[28] Karthik EVG, Priya V (2021) Gayathri. R, Dhanraj Ganapathy. Health Benefits Of Annona Muricata-A Review. Int J Dentistry Oral Sci 8:2965–2967

[29] Ganapathy D, (2021) Awareness of hazards caused by long-term usage of polyethylene terephthalate (PET) bottles. Int J Dent Oral Sci 2976–2980