Antioxidant Vitamins (Vit C and E) Protect the Kidney from Glyphosate- Exposed Renal Damage in Experimental Rats - A Molecular Study
Abstract:
Glyphosate is a widely used
herbicide and the active ingredient in Monsanto's Roundup, among other
herbicide formulations. Vitamin C (ascorbic acid) and vitamin E (tocopherol)
are both antioxidants that play important roles in reducing oxidative DNA
damage and protecting cells from the harmful effects of free radicals. The
study aimed to analyse the effect of antioxidant vitamins (vitamins C and E) on
the expression of IGF-1 and KIM-1 molecules in the kidneys of
glyphosate-exposed rats. Adult male Albino Wistar rats were grouped into 3. Group
I: Control; Group II: Glyphosate treated (100mg); Group III: Glyphosate+ vitamin
E & C treated. Serum, insulin, and mRNA expression of insulin-like growth
factor (IGF-1) and kidney injury molecules (KIM-1) mRNA expression by RT-PCR.
Serum insulin was measured by the ELISA method. Glyphosate-exposed rats
developed hyperglycaemia and hyper compared to control but antioxidant vitamins
(Vitamin C and E) supplementation, reduced the glyphosate mediate increased
fasting blood glucose and insulin near to that of the control level. Similarly,
mRNA expression of both IGF-1 and KIM-1 were also significantly altered due to
glyphosate induction whose effects were found to be reduced when vitamins C and
E were treated. It is concluded that glyphosate exposure causes renal damage
and develops diabetic nephropathy by modulating the expression of IGF-1 and
KIM-1 molecules in the kidney. Vitamin C and E potentially were able to reduce
the detrimental changes caused by glyphosate. Hence, vitamin C and E supplements
could be considered therapeutic drugs for diabetic nephropathy.
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