Abstract:

Periodontitis (PD) is a severe oral inflammation that tends to decay alveolar bone and loss of periodontal ligament. Also, ruptures of gingival tissues and pocket formation take place which can also impact various systematic health conditions. The present study was carried out to evaluate the impact of fasting and other treatment interventions in the prevention of ligature-induced periodontitis as well as the impact of these interventions in systemic inflammation in mice. Periodontitis was induced in mice through 3.0 silk ligature wire by passing between incisor teeth.  In this study, bacterial accumulation, bone loss, inflammatory markers such as TNF-α, IL-6, IL-1β, and C-reactive protein as well as oxidative stress i.e., TBARS, and antioxidant enzymes viz. GSH and catalase in dental tissue were measured. Additionally, mitochondrial enzyme complexes activity was significantly decreased in the periodontitis model which is significantly restored by the interventions applied in the study. Further histopathological analysis was also carried out in dental tissue. In periodontitis, there was a rise in bacterial accumulation and bone loss, as well as an increase in inflammatory markers, oxidative stress, and a decrease in mitochondrial enzyme complexes in gum tissue and antioxidative enzymes. Further, in ligature-induced periodontitis, infiltration of inflammatory cells along with the destruction of the cementum and the alveolar process was observed. However, these parameters were reversed after fasting and other treatment interventions. The current study reveals the following ligature removal and subsequent interventions including the impact of fasting to regenerate periodontal tissue.

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