Biogenic Selenium Nanoparticles Loaded Alginate-Gelatin Scaffolds for Potential Tissue Engineering Applications
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Abstract:
Selenium nanoparticles (SeNPs) were reported
for its anticancer and antimicrobial properties. Alginate and gelatin scaffolds
can act as an important biomaterial, more specifically in bone tissue engineering.
Green synthesis of SeNPs from Luffa cylindrica (LC) and loading of SeNPS with alginate-gelatin
scaffold and to check its biocompatibility. The SeNPs were prepared via the green
synthesis method and loaded into an alginate-gelatin scaffold. Characterization
studies such as UV-Vis spectroscopy, FTIR, and SEM were carried out in LC-SeNPs
and Se-NPs loaded scaffold. The hydrophilicity of the scaffolds was determined using
water contact angle measurements. Annexin V PI assay was conducted to determine
the biocompatible nature of prepared SeNPs-loaded alginate-gelatin scaffolds. The
UV-VIS spectrum gave an intense peak at 266 and 384 nm, whereas the FTIR gave a
strong peak at 3500-500 cm-1 fingerprint regions. SEM images showed flower-shaped
LC-SeNPs and their distribution of SeNPs on the surface of alginate-gelatin scaffolds.
water contact angle measurement was found to be 29.21°. Cell viability results showed
78.11% viable cells following treatment with alg-gel-Se scaffold, revealing its
biocompatibility towards peripheral blood mononuclear cells. Overall, it could be
concluded that the SeNP-loaded alg-gel scaffold is a promising candidate for tissue
engineering, but further studies are required to confirm its potential role.
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