Green Synthesis of Gold Nanoparticles Using Eucalyptus and Piper Longum and Its Subsequent Antiinflammatory Activity Evaluation
Abstract:
Gold is the oldest dental restorative material,
used for dental repairs for more than 4000 years and remains an important metal
included in the dental sector. In a world where nanoparticle importance has been well established and preparation
of nanoparticles has become much easier, it is important to assess if these nanoparticles
can be extracted from plants as well. Along with its extraction, analysis of each
property of the nanoparticle is essential. Pepper and eucalyptus remain two
of the most important ingredients used in ayurveda and can be easily found in every
household. The aim of this study was to extract gold nanoparticles using Eucalyptus
and Piper longum and evaluate the antibacterial activity of the derived gold nanoparticles.
Preparation of plant extract was done following which, extraction of gold nanoparticles
was performed. Antiinflammatory properties of the gold nanoparticles were tested
by albumin denaturation method and compared against the anti-inflammatory gold standard,
Diclofenac sodium. The protein denaturation levels were measured, and the data was
compiled. From this study, it
can be concluded that gold nanoparticles derived from pepper and eucalyptus can
be used as a potential source of anti-inflammatories.
References:
[1] Chen PC, Mwakwari SC, Oyelere AK. Gold nanoparticles:
from nanomedicine to nanosensing. Nanotechnology, science, and applications. 2008;
1:45.
[2] Gielen M, Tiekink ER, editors. Metallotherapeutic drugs
and metal-based diagnostic agents: the use of metals in medicine. John Wiley &
Sons; 2005 Sep 1.
[3] Kumar PS, Pastoriza-Santos I, Rodríguez-González B, De
Abajo FJ, Liz-Marzán LM. High-yield synthesis and optical response of gold nanostars.
Nanotechnology. 2007 Nov 29;19(1):015606.
[4] Edelman ER, Seifert P, Groothuis A, Morss A, Bornstein
D, Rogers C. Gold-coated NIR stents in porcine coronary arteries. Circulation. 2001
Jan 23;103(3):429-34.
[5] Svedman C, Tillman C, Gustavsson CG, Möller H, Frennby
B, Bruze M. Contact allergy to gold in patients with gold‐plated intracoronary stents.
Contact Dermatitis. 2005 Apr;52(4):192-6.
[6] Thelen A, Bauknecht HC, Asbach P, Schrom T. Behavior
of metal implants used in ENT surgery in 7 Tesla magnetic resonance imaging. European
Archives of Oto-Rhino-Laryngology and Head & Neck. 2006 Oct;263(10):900-5.
[7] Demann ET, Stein PS, Haubenreich JE. Gold as an implant
in medicine and dentistry. Journal of long-term effects of medical implants. 2005;15(6).
[8] Wersäll PJ, Blomgren H, Pisa P, Lax I, Kälkner KM, Svedman
C. Regression of non-irradiated metastases after extracranial stereotactic radiotherapy
in metastatic renal cell carcinoma. Acta oncologica. 2006 Jan 1;45(4):493-7.
[9] Shaw CF. Gold-based therapeutic agents. Chemical reviews.
1999 Sep 8;99(9):2589-600.
[10] Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, Wang H, Wang
Y, Shao W, He N, Hong J. Biosynthesis of silver and gold nanoparticles by novel
sundried Cinnamomum camphora leaf. Nanotechnology. 2007 Feb 6;18(10):105104.
[11] Wright DC, German RM, Gallant RF. Materials Science:
Copper and Silver Corrosion Activity in Crown and Bridge Alloys. Journal of Dental
Research. 1981 Apr;60(4):809-14.
[12] Knosp H, Holliday RJ, Corti CW. Gold in dentistry alloys,
uses and performance. Gold bulletin. 2003 Sep;36(3):93-102
[13] Rieshy, V., Priya, J., Arivarasu, L., Kumar, S. R.,
& Devi, G. (2020). Enhanced antimicrobial activity of herbal formulation
mediated copper nanoparticles against clinical pathogens. Plant cell biotechnology and molecular biology, 21(53-54), 52–56.
[14] Kishore, S. O. G., Priya, A. J., Narayanan, L.,
Kumar, S. R., & Devi, G. (2020). Controlling of oral pathogens using
turmeric and tulsi herbal formulation mediated copper nanoparticles. Plant cell biotechnology and molecular
biology, 21(53-54), 33–37.
[15] Sneka S, Preetha Santhakumar.
Antibacterial Activity of Selenium Nanoparticles extracted from Capparis decidua
against Escherichia coli and Lactobacillus Species. Research Journal of Pharmacy
and Technology. 2021; 14(8):4452-4. doi: 10.52711/0974-360X.2021.00773
[16] Roshan, A., Jothipriya, A., Arivarasu, L., Kumar,
R., & Devi, G. (2020). Antifungal activity of tulsi and turmeric assisted
copper nano particles. Plant cell
biotechnology and molecular biology, 21(27-28),
9–13.
[17] Iffat Nasim, S. Rajeshkumar, V Vishnupriya. Green Synthesis
of Reduced Graphene Oxide Nanoparticles, Its Characterization and Antimicrobial
Properties against Common Oral Pathogens. Int J Dentistry Oral Sci. 2021;8(2):1670-1675
[18] Nasim I, Kumar SR, Vishnupriya V, Jabin Z. Cytotoxicity
and anti-microbial analysis of silver and graphene oxide bio nanoparticles. Bioinformation.
2020;16(11):831.
[19] Rajeshkumar S, Lakshmi T. Anticariogenic Activity Of
Silver Nanoparticles Synthesized Using Fresh Leaves Extract Of Kalanchoe Pinnata.
Int J Dentistry Oral Sci. 2021 Jul 2;8(7):2985-7.
[20] Rajeshkumar S, Jayapriya J, Lakshmi T. A Review on plant
mediated selenium nanoparticles and its applications: Selenium nanoparticles. Journal
of Population Therapeutics and Clinical Pharmacology. 2021;28(2).
[21] Kamath KA, Nasim I, Rajeshkumar S. Evaluation of the
re-mineralization capacity of a gold nanoparticle-based dental varnish: An in vitro
study. Journal of conservative dentistry: JCD. 2020 Jul;23(4):390.
[22] Maliael MT, Jain RK, Srirengalakshmi M. Effect of nanoparticle
coatings on frictional resistance of orthodontic archwires: a systematic review
and meta-analysis. World. 2022;13(4).
[23] Chokkattu JJ, Mary DJ, Shanmugam R, Neeharika S. Embryonic
Toxicology Evaluation of Ginger-and Clove-mediated Titanium Oxide Nanoparticles-based
Dental Varnish with Zebrafish. The Journal of Contemporary Dental Practice. 2023
Mar 17;23(11):1157-62.
[24] NivedaRajeshwaran JR, Rajeshkumar S. Evaluation of Antioxidant
and Anti-Inflammatory Activity of Grape Seed Oil Infused with Silver Nano-particles
an In Vitro Study. Int J Dentistry Oral Sci. 2021 Jul 15;8(7):3318-22.
[25] S.Sushanthi,
Srisakthi Doraikannan, Meignana Arumugham Indiran, Pradeepkumar Rathinavelu, Rajeshkumar
S. Vernonia Amygdalina Mediated Copper Nanoparticles and their Characterization
and Antimicrobial Activity - An In vitro Study. Int J Dentistry Oral Sci. 2021;8(7):3330-3334.
doi: http://dx.doi.org/10.19070/2377-8075-2100067.
[26] S Rajeshkumar, T Lakshmi. Green Synthesis of Gold Nanoparticles
Using Kalanchoe Pinnata and Its Free Radical Scavenging Activity. Int J Dentistry
Oral Sci. 2021;8(7):2981-2984. doi: dx.doi.org/10.19070/2377-8075-21000606.
[27] Sahoo SK, Labhasetwar V. Nanotech approaches to drug
delivery and imaging. Drug discovery today. 2003 Dec 15;8(24):1112-20.
[28] de la Escosura-Muñiz A, Maltez-da Costa M, Sánchez-Espinel
C, Díaz-Freitas B, Fernández-Suarez J, González-Fernández Á, Merkoçi A. Gold nanoparticle-based
electrochemical magnetoimmunosensor for rapid detection of anti-hepatitis B virus
antibodies in human serum. Biosensors and Bioelectronics. 2010 Dec 15;26(4):1710-4.
[29] Kawasaki ES, Player A. Nanotechnology, nanomedicine,
and the development of new, effective therapies for cancer. Nanomedicine: Nanotechnology,
Biology and Medicine. 2005 Jun 1;1(2):101-9.
[30] Lee JH, Koo YK, Cho HW, Cha HJ, Shin DU, Oh TG, Lee SJ.
Cysteine-loaded pH-responsive liposome/gold nanoparticles as a time-temperature
indicator with instantaneous color change. Innovative Food Science & Emerging
Technologies. 2021 Oct 1; 73:102794.
[31] Gu YJ, Cheng J, Lin CC, Lam YW, Cheng SH, Wong WT. Nuclear
penetration of surface functionalized gold nanoparticles. Toxicology and applied
Pharmacology. 2009 Jun 1;237(2):196-204.
[32] Kumar SA, Peter YA, Nadeau JL. Facile biosynthesis, separation,
and conjugation of gold nanoparticles to doxorubicin. Nanotechnology. 2008 Nov 18;19(49):495101.
[33] Baron R, Šljukić B, Salter C, Crossley A, Compton RG.
Electrochemical detection of arsenic on a gold nanoparticle array. Russian Journal
of Physical Chemistry A. 2007 Sep;81(9):1443-7.
[34] Lalaoui N, Rousselot-Pailley P, Robert V, Mekmouche Y,
Villalonga R, Holzinger M, Cosnier S, Tron T, Le Goff A. Direct electron transfer
between a site-specific pyrene-modified laccase and carbon nanotube/gold nanoparticle
supramolecular assemblies for bioelectrocatalytic dioxygen reduction. Acs Catalysis.
2016 Mar 4;6(3):1894-900.
[35] Kumar G, Sahoo D. Effect of seaweed liquid extract on
growth and yield of Triticum aestivum var. Pusa Gold. Journal of applied phycology.
2011 Apr;23(2):251-5.
[36] Lim Soo P, Sidorov SN, Mui J, Bronstein LM, Vali H, Eisenberg
A, Maysinger D. Gold-labeled block copolymer micelles reveal gold aggregates at
multiple subcellular sites. Langmuir. 2007 Apr 24;23(9):4830-6.
[37] Ishida O, Maruyama K, Sasaki K, Iwatsuru M. Size-dependent
extravasation, and interstitial localization of polyethyleneglycol liposomes in
solid tumor-bearing mice. International journal of pharmaceutics. 1999 Nov 10;190(1):49-56.
[38] Zharov VP, Galitovskaya EN, Johnson C, Kelly T. Synergistic
enhancement of selective nanophotothermolysis with gold nanoclusters: potential
for cancer therapy. Lasers in Surgery and Medicine: The Official Journal of the
American Society for Laser Medicine and Surgery. 2005 Sep;37(3):219-26.
[39] Frens G. Controlled nucleation for the regulation of
the particle size in monodisperse gold suspensions. Nature physical science. 1973
Jan;241(105):20-2.
[40] Brown KR, Natan MJ. Hydroxylamine seeding of colloidal
Au nanoparticles in solution and on surfaces. Langmuir. 1998 Feb 17;14(4):726-8.
[41] Gusta LV, O’connor BJ, Gao YP, Jana S. A re-evaluation
of controlled freeze-tests and controlled environment hardening conditions to estimate
the winter survival potential of hardy winter wheats. Canadian journal of plant
Science. 2001 Apr 1;81(2):241-6.
[42] Busbee BD, Obare SO, Murphy CJ. An improved synthesis
of high‐aspect‐ratio gold nanorods. Advanced Materials. 2003 Mar 4;15(5):414-6.
[43] Sau TK, Murphy CJ. Seeded high yield synthesis of short
Au nanorods in aqueous solution. Langmuir. 2004 Jul 20;20(15):6414-20.
[44] Sun W, Arculus RJ, Kamenetsky VS, Binns RA. Release of
gold-bearing fluids in convergent margin magmas prompted by magnetite crystallization.
Nature. 2004 Oct;431(7011):975-8.
[45] Kundu S, Panigrahi S, Praharaj S, Basu S, Ghosh SK, Pal
A, Pal T. Anisotropic growth of gold clusters to gold nanocubes under UV irradiation.
Nanotechnology. 2007 Jan 12;18(7):075712.
[46] Mitra RN, Das PK. In situ preparation of gold nanoparticles
of varying shape in molecular hydrogel of peptide amphiphiles. The Journal of Physical
Chemistry C. 2008 Jun 5;112(22):8159-66.
[47] Pyrpassopoulos S, Niarchos D, Nounesis G, Boukos N, Zafiropoulou
I, Tzitzios V. Synthesis, and self-organization of Au nanoparticles. Nanotechnology.
2007 Nov 1;18(48):485604.
[48] P. Manonmani, M. Ramar, N. Geetha, M. Valan Arasu, R.
Rasik Erusan, R. Mari Selvam, J. Jerlin Sowmiya. Synthesis of silver nanoparticles
using natural products from Acalypha Indica (Kuppaimeni) and curcuma longa (Turmeric)
on antimicrobial activities. M Ramar, IJPRBS, 2015; Volume 4(1): 151-164
[49] J.R. Vane, R.M. Botting, New insight into the mode of
action of anti-inflammatory drugs, Inflamm. Res. 44 (1995) 1–10.
[50] E. Yesilada, O. Ustun, E. Sezik, Y. Takaishi, Y. Ono, G. Honda, Inhibitory effect of turkish folk remedies on inflammatory cytokines: Interleukins-1alpha, interleukins-1beta and tumour necrosis factor alpha, J. Ethnopharmacol. 58 (1997) 59–73.
[51] E.L. Opie, On the relation of necrosis and inflammation
to denaturation of proteins, J. Exp. Med. 115 (1962) 597-608.
[52] E. Umapathy, E.J. Ndebia, A. Meeme, B. Adam, P. Menziwa,
B.N. Nkeh-Chungag et al., An experimental evaluation of Albucasetosa aqueous extract on membrane stabilization, protein denaturation
and white blood cell migration during acute inflammation, J. Med. Plants Res. 4
(2010) 789-795.
[53] Mizushima Y. Screening test for antirheumatic drugs.
Lancet. 1966; 288:443.