Abstract:

Curcumin is a type of polyphenol phytochemical that is bright yellow in colour and produced by the plant Curcuma longa. Despite its pharmacological properties, curcumin has low bioavailability, poor solubility, and undergoes rapid degradation. Nanoparticles (NPs) are used as a nanocarrier for drug delivery, to improve the stability and pharmacokinetics of the drug. Therefore, by coating curcumin over selenium NPs (SeNPs), the bioactivity, bioavailability, stability, and may increase the solubility of curcumin of SeNPs. This study aimed to synthesize the SeNPs from Orthosiphon stamineus leaf extract and coat it with curcumin and to characterize it and check its biocompatibility. Biosynthesis of SeNPs was carried out using plant extract of Evolvulus alsinoides and characterized using UV spectrophotometer, FT-IR, and SEM. Annexin V PI apoptotic and Hemolytic assay were used for checking biocompatibility. The UV-Vis spectrum gave a strong peak at 265 and 423 nm at various time intervals, indicating the SeNPs formation. Similarly, FT-IR has strong absorption bands at 3279, 1284, 1072, 1028, and cm⁻¹ with wavelengths ranging from 4000-500 cm^-1. SEM analysis of biosynthesized SeNPs showed a spherical shape. Our results suggest that curcumin-coated SeNPs possess greater biocompatibility towards PBMCs which was evaluated by Annexin V - PI assay and erythrocytes by hemolytic studies. Curcumin-coated Selenium nanoparticles were successfully synthesized by the biological method using leaf extract of Orthosiphon stamineus and reported as biocompatible using Flow cytometry. But a more detailed study should be done for implementing it in tissue engineering.

References:

[1] Nabi, F., Arain, M. A., Hassan, F., Umar, M., Rajput, N., Alagawany, M., … Liu, J. 2020. Nutraceutical role of selenium nanoparticles in poultry nutrition: a review. Worlds Poult. Sci. J. 76 (3): 459–471.

[2] Pandiyan, I., Sri, S. D., Indiran, M. A., Rathinavelu, P. K., Prabakar, J., and Rajeshkumar, S. (2022). Antioxidant, anti-inflammatory activity of Thymus vulgaris-mediated selenium nanoparticles: An in vitro study. J. Conserv. Dent. 25 (3): 241–245.

[3] Sushanthi, S., Srisakthi, D., MeignanaArumugham, I., Pradeepkumar, R., and Rajeshkumar, S. 2021. Vernonia Amygdalina Mediated Copper Nanoparticles and its Characterization and Antimicrobial Activity - An In Vitro Study. Int J Dent. Oral Sci., 8 (7): 3330–3334.

[4] Maiyo, F., and Singh, M. 2017. Selenium nanoparticles: potential in cancer gene and drug delivery. Nanomedicine , 12 (9): 1075–1089.

[5] Maliael, M. T., Jain, R. K., and Srirengalakshmi, M. 2022. Effect of nanoparticle coatings on frictional resistance of orthodontic archwires: a systematic review and meta-analysis. World J. Dent, 13(4), 417–424.

[6] Nasim, I., Jabin, Z., Kumar, S. R., and Vishnupriya, V. (2022). Green synthesis of calcium hydroxide-coated silver nanoparticles using Andrographis paniculata and Ocimum sanctum Linn. leaf extracts: An antimicrobial and cytotoxic activity. JJ. Conserv. Dent. 25 (4): 369–374.

[7] Kamath, K. A., Nasim, I., and Rajeshkumar, S. (2020). Evaluation of the re-mineralization capacity of a gold nanoparticle-based dental varnish: An in vitro study. J. Conserv. Dent. 23 (4): 390–394.

[8] Maheswari, T. N. U., and Dhanvanth, M. 2022. Topical herbal therapeutic formulation used in the management of oral potentially malignant disorders – A systematic review. J. Indian Acad. Oral Med. Radiol. 34 (2): 223.

[9] Ghaderi, R. S., Adibian, F., Sabouri, Z., Davoodi, J., Kazemi, M., Amel Jamehdar, S., … Daroudi, M. 2022. Green synthesis of selenium nanoparticle by Abelmoschus esculentus extract and assessment of its antibacterial activity. Mater. Tech. 37(10), 1289–1297.

[10] Deng, W., Xie, Q., Wang, H., Ma, Z., Wu, B., and Zhang, X. 2017. Selenium nanoparticles as versatile carriers for oral delivery of insulin: Insight into the synergic antidiabetic effect and mechanism. Nanomed.: Nanotechnol. Biol. Med. 13 (6): 1965–1974.

[11] Pyrzynska, K., and Sentkowska, A. 2022. Biosynthesis of selenium nanoparticles using plant extracts. J. Nanostructure Chem.12 (4): 467–480.

[12] Sentkowska, A., and Pyrzyńska, K. 2022. The Influence of Synthesis Conditions on the Antioxidant Activity of Selenium Nanoparticles. Molecules , 27 (8): 2486

[13] Shirmehenji, R., Javanshir, S., and Honarmand, M. 2021. A Green Approach to the Bio-based Synthesis of Selenium Nanoparticles from Mining Waste. J. Cluster Sci. 32 (5): 1311–1323.

[14] Kora, A. J., and Rastogi, L. 2016. Biomimetic synthesis of selenium nanoparticles by Pseudomonas aeruginosa ATCC 27853: An approach for conversion of selenite. J. Environ. Manage. 181: 231–236.

[15] Meenambigai, K., Kokila, R., Chandhirasekar, K., Thendralmanikandan, A., Kaliannan, D., Ibrahim, K. S., … Nareshkumar, A. 2022. Green Synthesis of Selenium Nanoparticles Mediated by Nilgirianthus ciliates Leaf Extracts for Antimicrobial Activity on Foodborne Pathogenic Microbes and Pesticidal Activity Against Aedes aegypti with Molecular Docking. Biol. Trace Elem. Res. 200 (6): 2948–2962.

[16] Hatami, R., Javadi, A., & Jafarizadeh-Malmiri, H. (2020). Effectiveness of six different methods in green synthesis of selenium nanoparticles using propolis extract: Screening and characterization. Green Processing and Synthesis, 9(1), 685–692.

[17] Johnson, J., Shanmugam, R., & Lakshmi, T. (2022). A review on plant-mediated selenium nanoparticles and its applications. J. Popul. Ther. Clin. Pharmacol. 28 (2): e29–e40.

[18] Mi, X.-J., Choi, H. S., Perumalsamy, H., Shanmugam, R., Thangavelu, L., Balusamy, S. R., and Kim, Y.-J. 2022. Biosynthesis and cytotoxic effect of silymarin-functionalized selenium nanoparticles induced autophagy mediated cellular apoptosis via downregulation of PI3K/Akt/mTOR pathway in gastric cancer. Phytomedicine, 99: 154014.

[19] Ezhilarasan, D., Lakshmi, T., and Mallineni, S. K. 2022. Nano-based targeted drug delivery for lung cancer: therapeutic avenues and challenges. Nanomedicine , 17 (24): 1855–1869.

[20] Safaei, M., Mozaffari, H. R., Moradpoor, H., Imani, M. M., Sharifi, R., and Golshah, A. 2022. Optimization of Green Synthesis of Selenium Nanoparticles and Evaluation of Their Antifungal Activity against Oral Candida albicans Infection. Adv. Mater. Sci. Eng. 2022: 1376998

[21] Liu, W., Li, X., Wong, Y.-S., Zheng, W., Zhang, Y., Cao, W., and Chen, T. 2012. Selenium nanoparticles as a carrier of 5-fluorouracil to achieve anticancer synergism. ACS nano, 6 (8): 6578–6591.

[22] Nasim, I., Rajesh Kumar, S., Vishnupriya, V., and Jabin, Z. 2020. Cytotoxicity and anti-microbial analysis of silver and graphene oxide bio nanoparticles. Bioinformation, 16 (11): 831–836.

[23] Nasim, I., Rajeshkumar, S., and Vishnupriya, V. 2021. Green synthesis of reduced graphene oxide nanoparticles, its characterization, and antimicrobial properties against common oral pathogens. Int J Dentistry Oral Sci., 8 (2): 1670–1675.

[24] Kishore, S. O. G., Priya, A. J., and Narayanan, L. 2020. Controlling of oral pathogens using turmeric and tulsi herbal formulation mediated copper nanoparticles. Plant Cell Biotechnol. Mol. Biol. 21 (53-54): 33-37

[25] Rieshy, V., Priya, J., Arivarasu, L., and Kumar, S. R. 2020. Enhanced Antimicrobial Activity of Herbal Formulation Mediated Copper Nanoparticles Against Clinical Pathogens. Plant Cell Biotechnol. Mol. Biol. 21 (53-54): 52-56.

[26] Sneka, and Santhakumar, P. 2021. Antibacterial Activity of Selenium Nanoparticles extracted from Capparis decidua against Escherichia coli and Lactobacillus Species. J. Adv. Pharm. Tech. Res. 14 (8): 4452–4454.

[27] Palombo, E. A. 2011. Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases. Evid. Based. Complement. Alternat. Med. 2011: 680354.

[28] Rajeshkumar, S., Lakshmi, T., and Tharani, M. (2021). Green synthesis of copper nanoparticles synthesized using black tea and its antibacterial activity against oral pathogens. Int. J. Dent. Oral Sci., 8 (9): 4156–4159.

[29] Rajeshkumar, S., and Lakshmi, S. 2021. Anticariogenic activity of silver nanoparticles synthesized using fresh leaves extract of kalanchoe pinnata. . Int J Dentistry Oral Sci., 8 (7): 2985–2987.

[30] Twaij, B. M., and Hasan, M. N. 2022. Bioactive Secondary Metabolites from Plant Sources: Types, Synthesis, and Their Therapeutic Uses. Int. J.Plant Biol. 13 (1): 4–14.

[31] Sharifi-Rad, J., Rayess, Y. E., Rizk, A. A., Sadaka, C., Zgheib, R., Zam, W., … Martins, N. 2020. Turmeric and Its Major Compound Curcumin on Health: Bioactive Effects and Safety Profiles for Food, Pharmaceutical, Biotechnological and Medicinal Applications. Front. Pharmacol. 11: 01021.

[32] Ashraf, K., Sultan, S., and Adam, A. 2018. Orthosiphon stamineus Benth. is an Outstanding Food Medicine: Review of Phytochemical and Pharmacological Activities. J. Pharm. Bioallied Sci. 10 (3): 109–118.

[33] Wang, Q., Wang, J., Li, N., Liu, J., Zhou, J., Zhuang, P., and Chen, H. 2022. A Systematic Review of Orthosiphon stamineus Benth. in the Treatment of Diabetes and Its Complications. Molecules. 27 (2): 444

[34] Hossain, M. A., Ismail, Z., Rahman, A., and Kang, S. C. 2008. Chemical composition and anti-fungal properties of the essential oils and crude extracts of Orthosiphon stamineus Benth. Ind. Crops Prod. 27 (3): 328–334.

[35] Hossain, M. A., and Mizanur Rahman, S. M. 2015. Isolation and characterisation of flavonoids from the leaves of medicinal plant Orthosiphon stamineus. Arab. J. Chem. 8 (2): 218–221.

[36] Ameer, O. Z., Salman, I. M., Asmawi, M. Z., Ibraheem, Z. O., and Yam, M. F. 2012. Orthosiphon stamineus: traditional uses, phytochemistry, pharmacology, and toxicology. J. Med. Food. 15 (8):, 678–690.

[37] Guo, M., Li, Y., Lin, Z., Zhao, M., Xiao, M., Wang, C., … Zhu, B. 2017. Surface decoration of selenium nanoparticles with curcumin induced HepG2 cell apoptosis through ROS mediated p53 and AKT signaling pathways. RSC Adv. 7 (83): 52456–52464.

[38] Francis, S., Joseph, S., Koshy, E. P., and Mathew, B. 2017. Green synthesis and characterization of gold and silver nanoparticles using Mussaenda glabrata leaf extract and their environmental applications to dye degradation. Environ. Sci. Pollut. Res. 24 (21): 17347–17357.

[39] Bulmus, V., Woodward, M., Lin, L., Murthy, N., Stayton, P., and Hoffman, A. 2003. A new pH-responsive and glutathione-reactive, endosomal membrane-disruptive polymeric carrier for intracellular delivery of biomolecular drugs. J. Control. Release. 93(2), 105–120.

[40] Qi, B., Wang, C., Ding, J., and Tao, W. (2019). Editorial: Applications of Nanobiotechnology in Pharmacology. Front.  Pharmacol. 10: 1451.

[41] Cittrarasu, V., Kaliannan, D., Dharman, K., Maluventhen, V., Easwaran, M., Liu, W. C.,
Arumugam, M. 2021. Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Sci. Rep. 11 (1): 1032.

[42] Gunti, L., Dass, R. S., and Kalagatur, N. K. 2019. Phytofabrication of Selenium Nanoparticles from Emblica officinalis Fruit Extract and Exploring Its Biopotential Applications: Antioxidant, Antimicrobial, and Biocompatibility. Front. Microbiol. 10: 931.

[43] Ramamurthy, C. H., Sampath, K. S., Arunkumar, P., Suresh Kumar, M., Sujatha, V., Premkumar, K., and Thirunavukkarasu, C. 2013. Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst. Eng. 36 (8): 1131-1139.