Abstract:

A stem cell is a master of all cells in the body, which can divide and regrow with regeneration and vascularization. The deciduous teeth, which are not infected and vital with pulp stem cells, help in the formation of adult stem cells. The extracted stem cells from human exfoliated deciduous teeth (SHED) and immature dental pulp stem cells can be harvested as stem cells that form dental tissue regeneration SHED, osteogenic potential 40%, and neurogenic potential either by in vivo or in vitro method. The article here focuses mainly on the stem cell classification of dental stem cells, their characterization, therapeutic application, application in the deciduous tooth procedure, banking of SHED, and recent innovative methods for banking SHED. This review was done to highlight the integral role of pedodontists in educating patients about stem cell banking services and must become a part of such services.

References:

[1] Stem Cells: Past, Present, and the Future https://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1165-5.

[2] Stem Cells: https://medlineplus.gov/stemcells.html.

[3] Miura, M., Gronthos, S., Zhao, M., Lu, B., Fisher, L. W., Robey, P. G., & Shi, S., 2003, SHED: stem cells from human exfoliated deciduous teeth. Proceedings of the National Academy of Sciences of the United States of America, 100(10), 5807–5812.

[4] Kerkis, I., Kerkis, A., Dozortsev, D., Stukart-Parsons, G. C., Gomes Massironi, S. M., Pereira, L. V., Caplan, A. I., & Cerruti, H. F., 2006, Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. Cells, tissues, organs, 184(3-4), 105–116.

[5] Popuri S. K., 2018, Concerns of a Pediatric Dentist in Dental Stem Cells: An Overview. The Open Dentistry Journal, 12, 596–604.

[6] Kerkis, I., & Caplan, A. I., 2012, Stem cells in dental pulp of deciduous teeth. Tissue Engineering. Part B, Reviews, 18(2), 129–138.

[7] Saber S. E., 2009, Tissue engineering in endodontics. Journal of Oral Science, 51(4), 495–507.

[8] Cordeiro, M. M., Dong, Z., Kaneko, T., Zhang, Z., Miyazawa, M., Shi, S., Smith, A. J., & Nör, J. E., 2008, Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. Journal of Endodontics, 34(8), 962–969.

[9] Bansal, R., & Jain, A., 2015, Current overview on dental stem cells applications in regenerative dentistry. Journal of Natural Science, biology, and medicine, 6(1), 29–34.

[10] de Mendonça Costa, A., Bueno, D. F., Martins, M. T., Kerkis, I., Kerkis, A., Fanganiello, R. D., Cerruti, H., Alonso, N., & Passos-Bueno, M. R., 2008, Reconstruction of large cranial defects in nonimmunosuppressed experimental design with human dental pulp stem cells. The Journal of Craniofacial Surgery, 19(1), 204–210.

[11] Papaccio, G., Graziano, A., d’Aquino, R., Graziano, M. F., Pirozzi, G., Menditti, D., De Rosa, A., Carinci, F., & Laino, G., 2006, Long-term cryopreservation of dental pulp stem cells (SBP-DPSCs) and their differentiated osteoblasts: a cell source for tissue repair. Journal of Cellular Physiology, 208(2), 319–325.

[12] Rosa, V., Dubey, N., Islam, I., Min, K. S., & Nör, J. E., 2016, Pluripotency of Stem Cells from Human Exfoliated Deciduous Teeth for Tissue Engineering. Stem Cells International, 2016, 5957806.

[13] Arora, V., Arora, P., & Munshi, A. K., 2009, Banking stem cells from human exfoliated deciduous teeth (SHED): saving for the future. The Journal of Clinical Pediatric Dentistry, 33(4), 289–294.

[14] Telles, P. D., Machado, M. A., Sakai, V. T., & Nör, J. E., 2011, Pulp tissue from primary teeth: new source of stem cells. Journal of Applied Oral Science: Revista FOB, 19(3), 189–194.

[15] Friedlander, L. T., Cullinan, M. P., & Love, R. M., 2009, Dental stem cells and their potential role in apexogenesis and apexification. International Endodontic Journal, 42(11), 955–962.

[16] Caruso, S., Sgolastra, F., & Gatto, R., 2014, Dental pulp regeneration in paediatric dentistry: the role of stem cells. European Journal of Paediatric Dentistry, 15(2), 90–94.

[17] Chrepa, V., Pitcher, B., Henry, M. A., & Diogenes, A., 2017, Survival of the Apical Papilla and Its Resident Stem Cells in a Case of Advanced Pulpal Necrosis and Apical Periodontitis. Journal of Endodontics, 43(4), 561–567.

[18] Tsai, A. I., Hong, H. H., Lin, W. R., Fu, J. F., Chang, C. C., Wang, I. K., Huang, W. H., Weng, C. H., Hsu, C. W., & Yen, T. H., 2017, Isolation of Mesenchymal Stem Cells from Human Deciduous Teeth Pulp. BioMed Research International, 2017, 2851906.

[19] Yin, Z., Wang, Q., Li, Y., Wei, H., Shi, J., & Li, A., 2016, A novel method for banking stem cells from human exfoliated deciduous teeth: lentiviral TERT immortalization and phenotypical analysis. Stem Cell Research & Therapy, 7, 50.

[20] Narang, S., & Sehgal, N., 2012, Stem cells: A potential regenerative future in dentistry. Indian Journal of Human Genetics, 18(2), 150–154.