Leptospirosis Diagnostic Capacity in Public Health Facilities within Nairobi County, Kenya
Abstract:
The presence
of biotic and abiotic reservoirs is a possible indicator of leptospirosis occurrence
in Nairobi County, which has a large proportion of informal settlements. Despite
these epidemiological risk factors, little or no attention is accorded to leptospirosis.
The study assessed the degree of leptospirosis diagnostic capacity in public health
facilities within Nairobi County, Kenya. A descriptive cross-sectional study involving
133 clinicians and 15 laboratory personnel across 15 public health facilities was
conducted between August and December 2019. The perception of zoonotic management
was high as 95% (126) had a strong score while 5% (7) had a weak score. Healthcare
facility level (χ2 (3) = 14.09, p < 0.05), professional designation (χ2 (1) =
4.26, p < 0.05) had a significant association with suspecting zoonosis. The length
of service as a clinician was a significant predictor of suspecting zoonosis, Wald
= 11.11, p < 0.05. Inter-agency collaboration was low as 89% (119) reported a
lack of sharing zoonosis information, and only 8% (10) indicated that there was
information sharing. The clinical suspicion index was low, 3.8% of the participants
suspected the disease in practice, and 2.3% would consider leptospirosis in the
differential diagnosis of FUO. All 15 public health facilities lacked leptospirosis
laboratory diagnostic capacity. The probable diagnosis of leptospirosis is low due
to a low clinical suspicion index and lack of awareness. There is a lack of laboratory
diagnostic capacity. Sensitisation of clinicians and laboratory personnel is critical
in increasing the diagnostic capacity of leptospirosis.
References:
[1] Haake, D. A., & Levett, P. N. (2014). Leptospirosis
in humans. Current Topics in Microbiology and Immunology, 65-97. https://doi.org/10.1007/978-3-662-45059-8_5.
[2] Costa, F., Hagan, J. E., Calcagno, J., Kane, M., Torgerson,
P., Martinez-Silveira, M. S., Stein, C., Abela-Ridder, B., & Ko, A. I. (2015).
Global morbidity and mortality of leptospirosis: A systematic review. PLOS Neglected
Tropical Diseases, 9(9), e0003898. https://doi.org/10.1371/journal.pntd.0003898.
[3] Wuthiekanun, V., Sirisukkarn, N., Daengsupa, P., Sakaraserane,
P., Sangkakam, A., Chierakul, W., Smythe, L. D., Symonds, M. L., Dohnt, M. F., Slack,
A. T., Day, N. P., & Peacock, S. J. (2007). Clinical diagnosis and geographic
distribution of leptospirosis, Thailand. Emerging Infectious Diseases, 13(1), 124-126.
https://doi.org/10.3201/eid1301.060718.
[4] Mgode, G. F., Machang’u, R. S., Mhamphi, G. G., Katakweba,
A., Mulungu, L. S., Durnez, L., Leirs, H., Hartskeerl, R. A., & Belmain, S.
R. (2015). Leptospira Serovars for diagnosis of leptospirosis in humans and animals
in Africa: Common leptospira isolates and reservoir hosts. PLOS Neglected Tropical
Diseases, 9(12), e0004251. https://doi.org/10.1371/journal.pntd.0004251.
[5] Halliday, J. E., Allan, K. J., Ekwem, D., Cleaveland, S.,
Kazwala, R. R., & Crump, J. A. (2015). Endemic zoonoses in the tropics: A public
health problem hiding in plain sight. Veterinary Record, 176(9), 220-225. https://doi.org/10.1136/vr.h798.
[6] Gebreyes, W. A., Dupouy-Camet, J., Newport, M. J., Oliveira,
C. J., Schlesinger, L. S., Saif, Y. M., Kariuki, S., Saif, L. J., Saville, W., Wittum,
T., Hoet, A., Quessy, S., Kazwala, R., Tekola, B., Shryock, T., Bisesi, M., Patchanee,
P., Boonmar, S., & King, L. J. (2014). The global one health paradigm: Challenges
and opportunities for tackling infectious diseases at the human, animal, and environment
interface in low-resource settings. Plos Neglected Tropical Diseases, 8(11),
e3257. https://doi.org/10.1371/journal.pntd.0003257.
[7] Halliday, J., Daborn, C., Auty, H., Mtema, Z., Lembo, T.,
Bronsvoort, B. M., Handel, I., Knobel, D., Hampson, K., & Cleaveland, S. (2012).
Bringing together emerging and endemic zoonoses surveillance: Shared challenges
and a common solution. Philosophical Transactions of the Royal Society B: Biological
Sciences, 367(1604), 2872-2880. https://doi.org/10.1098/rstb.2011.0362.
[8] Adler, B.,
& de la Peña Moctezuma, A. (2010). Leptospira and leptospirosis. Veterinary
Microbiology, 140(3-4), 287-296. https://doi.org/10.1016/j.vetmic.2009.03.012.
[9] De Brito, T., Silva, A. M., & Abreu, P. A. (2018).
Pathology and pathogenesis of human leptospirosis: A commented review. Revista
do Instituto de Medicina Tropical de São Paulo, 60(0). https://doi.org/10.1590/s1678-9946201860023.
[10] Faisal, S. M., McDonough, S. P., & Chang, Y. (2012).
Leptospira: Invasion, pathogenesis and persistence. The Pathogenic Spirochetes:
strategies for evasion of host immunity and persistence, 143-172. https://doi.org/10.1007/978-1-4614-5404-5_88.
[11] Crump, J. A., Morrissey, A. B., Nicholson, W. L., Massung,
R. F., Stoddard, R. A., Galloway, R. L., Ooi, E. E., Maro, V. P., Saganda, W., Kinabo,
G. D., Muiruri, C., & Bartlett, J. A. (2013). Etiology of severe non-malaria
febrile illness in northern Tanzania: A prospective cohort study. PLoS Neglected
Tropical Diseases, 7(7), e2324. https://doi.org/10.1371/journal.pntd.0002324.
[12] Biggs, H. M., Hertz, J. T., Munishi, O. M., Galloway, R.
L., Marks, F., Saganda, W., Maro, V. P., & Crump, J. A. (2013). Estimating leptospirosis
incidence using hospital-based surveillance and a population-based health care utilization
survey in Tanzania. PLoS Neglected Tropical Diseases, 7(12), e2589. https://doi.org/10.1371/journal.pntd.0002589.
[13] Fischer, R. S., & Flores Somarriba, B. (2017). Challenges
to diagnosing leptospirosis in endemic regions require urgent attention. Current
Tropical Medicine Reports, 4(2), 57-61. https://doi.org/10.1007/s40475-017-0110-x.
[14] Destoumieux-Garzón, D., Mavingui, P., Boetsch, G., Boissier,
J., Darriet, F., Duboz, P., Fritsch, C., Giraudoux, P., Le Roux, F., Morand, S.,
Paillard, C., Pontier, D., Sueur, C., & Voituron, Y. (2018). The one health
concept: 10 Years old and a long road ahead. Frontiers in Veterinary Science,
5. https://doi.org/10.3389/fvets.2018.00014.
[15] llan, K. J., Biggs, H. M., Halliday, J. E., Kazwala, R.
R., Maro, V. P., Cleaveland, S., & Crump, J. A. (2015). Epidemiology of leptospirosis
in Africa: A systematic review of a neglected zoonosis and a paradigm for ‘One health’
in Africa. PLOS Neglected Tropical Diseases, 9(9), e0003899. https://doi.org/10.1371/journal.pntd.0003899.
[16] Lau, C. L., Smythe, L. D., Craig, S. B., & Weinstein,
P. (2010). Climate change, flooding, urbanisation and leptospirosis: Fuelling the
fire? Transactions of the Royal Society of Tropical Medicine and Hygiene, 104(10),
631-638. https://doi.org/10.1016/j.trstmh.2010.07.002.
[17] Torgerson, P. R., Hagan, J. E., Costa, F., Calcagno, J.,
Kane, M., Martinez-Silveira, M. S., Goris, M. G., Stein, C., Ko, A. I., & Abela-Ridder,
B. (2015). Global burden of leptospirosis: Estimated in terms of disability adjusted
life years. PLOS Neglected Tropical Diseases, 9(10), e0004122. https://doi.org/10.1371/journal.pntd.0004122.
[18] Musso, D., & La Scola, B. (2013). Laboratory diagnosis
of leptospirosis: A challenge. Journal of Microbiology, Immunology and Infection,
46(4), 245-252. https://doi.org/10.1016/j.jmii.2013.03.001.
[19] Durski, K., Jancloes, M., Chowdhary, T., & Bertherat,
E. (2014). A global, multi-disciplinary, multi-sectorial initiative to combat leptospirosis:
Global leptospirosis environmental action network (GLEAN). International Journal
of Environmental Research and Public Health, 11(6), 6000-6008. https://doi.org/10.3390/ijerph110606000.
[20] De Vries, S. G., Visser, B. J., Nagel, I. M., Goris, M.
G., Hartskeerl, R. A., & Grobusch, M. P. (2014). Leptospirosis in sub-Saharan
Africa: A systematic review. International Journal of Infectious Diseases,
28, 47-64. https://doi.org/10.1016/j.ijid.2014.06.013.
[21] Baer, R., Turnberg, W., Yu, D., & Wohrle, R. (2009).
Leptospirosis in a small animal veterinarian: Reminder to follow standardized infection
control procedures. Zoonoses and Public Health, 57(4), 281-284. https://doi.org/10.1111/j.1863-2378.2009.01240.x.
[22] Bett, B., Said, M. Y., Sang, R., Bukachi, S., Wanyoike,
S., Kifugo, S. C., Otieno, F., Ontiri, E., Njeru, I., Lindahl, J., & Grace,
D. (2017). Effects of flood irrigation on the risk of selected zoonotic pathogens
in an arid and semi-arid area in the eastern Kenya. Plos One, 12(5), e0172626.
https://doi.org/10.1371/journal.pone.0172626.
[23] Cook, E. A., De Glanville, W. A., Thomas, L. F., Kariuki,
S., Bronsvoort, B. M., & Fèvre, E. M. (2016). Risk factors for leptospirosis
seropositivity in slaughterhouse workers in western Kenya. Occupational and Environmental
Medicine, 74(5), 357-365. https://doi.org/10.1136/oemed-2016-103895.
[24] Munyua, P., Bitek, A., Osoro, E., Pieracci, E. G., Muema,
J., Mwatondo, A., Kungu, M., Nanyingi, M., Gharpure, R., Njenga, K., & Thumbi,
S. M. (2016). Prioritization of zoonotic diseases in Kenya, 2015. Plos One, 11(8),
e0161576. https://doi.org/10.1371/journal.pone.0161576.
[25] De Geus, A., Wolff, J. W., & Timmer, V. E. A. (1977).
Clinical leptospirosis in Kenya, II. A field study in Nyanza Province. East African
medical journal, 54(3), 125-132. https://www.cabdirect.org/cabdirect/abstract/19792903083.
[26] Wachira, B. W., & Smith, W. (2013). Major incidents
in Kenya: The case for emergency services development and training. Prehospital
and Disaster Medicine, 28(2), 170-173. https://doi.org/10.1017/s1049023x13000010
[27] Halliday, J. E., Knobel, D. L., Allan, K. J., De C. Bronsvoort,
B. M., Handel, I., Agwanda, B., Cutler, S. J., Olack, B., Ahmed, A., Hartskeerl,
R. A., Njenga, M. K., Cleaveland, S., & Breiman, R. F. (2013). Urban leptospirosis
in Africa: A cross-sectional survey of leptospira infection in rodents in the Kibera
urban settlement, Nairobi, Kenya. The American Journal of Tropical Medicine and
Hygiene, 89(6), 1095-1102. https://doi.org/10.4269/ajtmh.13-0415.
[28] Harris, P. A., Taylor, R., Thielke, R., Payne, J., Gonzalez,
N., & Conde, J. G. (2009). Research electronic data capture (Redcap)—A metadata-driven
methodology and workflow process for providing translational research informatics
support. Journal of Biomedical Informatics, 42(2), 377-381. https://doi.org/10.1016/j.jbi.2008.08.010.
[29] Slotved, H., Yatich, K. K., Sam, S. O., & Ndhine, E.
O. (2017). The capacity of diagnostic laboratories in Kenya for detecting infectious
diseases. Tropical Medicine and Health, 45(1). https://doi.org/10.1186/s41182-017-0049-6.
[30] Bandara, K. K., Gunasekara, C. P., Weerasekera, M. M.,
Ranasinghe, N., Hapugoda, M., Marasinghe, C., & Fernando, N. (2016). Comparison
of three rapid diagnostic assays for diagnosis of leptospirosis in a resource-poor
setting. https://doi.org/10.20959/wjpr20167-6634.
[31] Kumar, S. S. (2013). Indian guidelines for the diagnosis
and management of human leptospirosis. Medicine Update, 2013.
[32] Ahmad, S. N., Shah, S., & Ahmad, F. H. (2005). Laboratory
diagnosis of leptospirosis. Journal of postgraduate medicine, 51(3), 195.
https://www.jpgmonline.com/article.asp?issn=0022-3859;year=2005;volume=51;issue=3;spage=195;epage=200;aulast=Ahmad.
[33] Gunasekara, C. P., Sumaiha, M. H. F., Damayanthi, M. K.
S., Weerasekara, M. M., & Fernando, S. S. N. (2017). Utility of a modified silver
staining technique for detection of Leptospira. https://sljid.sljol.info/articles/abstract/10.4038/sljid.v7i2.8142/.
[34] Mohamad Safiee, A. W., Mohd Ali, M. R., Fauzi, M. H., Muhd
Besari, A., Yean Yean, C., Neela, V. K., & Ismail, N. (2020). Leptospiral Culture
without 5’-Fluorouracil Revealed Improved Leptospira Isolation from Febrile Patients
in North-Eastern Malaysia. International journal of environmental research and
public health, 17(4), 1307. https://doi.org/10.3390/ijerph17041307
[35] Rao, M., Amran, F., & Aqilla, N. (2019). Evaluation
of a rapid kit for detection of IgM against Leptospira in human. Canadian Journal
of Infectious Diseases and Medical Microbiology, 2019. https://doi.org/10.1155/2019/5763595.
[36] Odhiambo, F., Galgalo, T., Wences, A., Muchemi, O. M.,
Kanyina, E. W., Tonui, J. C., ... & Boru, W. (2014). Antimicrobial resistance:
capacity and practices among clinical laboratories in Kenya, 2013. The Pan African
Medical Journal, 19. https://doi.org/10.11604/pamj.2014.19.332.5159.
[37] Kariuki, S., & Dougan, G. (2014). Antibacterial resistance
in sub-Saharan Africa: an underestimated emergency. Annals of the New York Academy
of Sciences, 1323(1), 43. https://doi.org/10.1111/nyas.12380.
[38] Onsare, R. S., Micoli, F., Lanzilao, L., Alfini, R., Okoro,
C. K., Muigai, A. W., ... & Rondini, S. (2015). Relationship between antibody
susceptibility and lipopolysaccharide O-antigen characteristics of invasive and
gastrointestinal nontyphoidal Salmonellae isolates from Kenya. Plos neglected
tropical diseases, 9(3), e0003573. https://doi.org/10.1371/journal.pntd.0003573.