Abstract:

Staphylococci represent the most commonly encountered blood culture isolates from ICU patients. Slime production is considered to be a significant virulence factor for some strains of Staphylococci. The importance of the role played by slime is further increased by its frequent association to reduced antibiotic susceptibility. This study was done to find out relationship of Bio-Film formation on the susceptibility pattern of the isolates. 27.6% of the isolates produced biofilm as detected by Congo Red Agar method; commonest being Staphylococcus epidermidis (53.9%). The percentage of resistance in the group which produces biofilm is always greater than the group which does not produce biofilm. Odds ratio for overall drug resistance was 2.10 (with 95% confidence interval as [1.6 – 2.6]) and with a p-value of 0.006.

References:

[1]. Morales Serrano T, Ramos S, Lara Gonzalez Y, Torres Colberg H, Vera Quiñones A, Miranda Santiago R, Amill S, Otero M, Cintron V, Villarreal Morales ML, ' Manatí Medical Center Sepsis Management Epidemiological Study', Bol Asoc Med P R (2015), Vol.107 No.2 pp.34-39.

[2]. Weinstein M P, Towns M L, Quartey S M, Mirrett S, Reimer L G, Parmigiani G, 'The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of the microbiology, epidemiology, and outcome of bacteremia and fungemia in adults', Clin Infect Dis. (1997), Vol.24 No.4 pp.584-602.

[3]. Bates D W, Sands K, Miller E, 'Predicting bacteremia in patients with sepsis syndrome. Academic Medical Center Consortium Sepsis Project Working Group', J Infect Dis (1997), Vol.176 pp.1538–1551.

[4]. Aygen B, Yoruk A, Yyldyz O, 'Bloodstream infections caused by Staphylococcus aureus in university hospital in Turkey: clinical and molecular epidemiology of methicillin-resistant Staphylococcus aureus', Clin Microbiol. Infect. (2004), Vol.10 pp.309–314.

[5]. Mathur P, Kapil A, Dan B, 'Nosocomial bacteremia in intensive care unit patients of a tertiary care center', Indian J Med Res. (2005), Vol.122 pp.305–308.

[6]. Ammendolia MG, Di Rosa R, Montanaro R, Arciola CR, Baldassarri L, 'Slime production and expression of the slime-associated antigen by Staphylococal clinical isolates', J Clin Microbiol (1999) Vol.37 pp.3235-3238.

[7]. Amorena B, Garcia E, Monzon M, Leiva J, Oteiza C, Perez M, 'Antibiotic susceptibility assay for Staphylococcus aureus in biofilms developed in-vitro', J. Antimicrob. Chemother. (1999), Vol.44 pp.43 – 55.

[8]. Kotulová D, Slobodníková L, 'Susceptibility of Staphylococcus aureus biofilms to Vancomycin, Gentamicin and Rifampin', Epidemiol Mikrobiol Imunol. (2010), Vol.59 No.2 pp.80-87.