Mycobacterium Tuberculosis (Mtb) Detection using Zielh-Neelson (Z-N) Stain to Identify Acid Fast Bacilli.
Abstract:
The
review of the article identifies Tuberculosis (TB) as an infectious disease
which is known worldwide with reference to India being the second largest
populated country in the world. Secondly it identifies Acid Fast Bacilli using
Zielh-Neelson (Z-N) staining in a clinical laboratory for the identification of
TB using the best possible method to obtain secretions from the lower airways
of patients as compared to bacterial identification which is normally used.
Various
diagnostic methods and materials used to evaluate the article authority and
accuracy will be outlined which includes sample study, period of examination,
ethical practices prior starting the study, study protocol, study design,
inclusion and exclusion criteria carried out systematically under sterile
conditions.
Analysis of AFB
smear will be explained and presented using tables which are instrumental in
the identification of other diagnostic methods like sputum smear microscopy and
X-ray method. Conclusion of the study will be based on the safest method for
identification of the organism, which is cost effective and has the potential
to offer treatment to the suspected patients. On the other hand, the article
has obtained its objectivity and coverage by using various ethical committees
and by declaring no conflict of interest among the authors who have contributed
equally to the study.
Finally, the
evaluation of the article will be carried through the use of well designed and
précised questionnaires, a case sheet proforma plus relevant referees attached
to the article to aid more understanding.
References:
[1.] Andrew, L., diagnostic surgical pathology of the head
and neck, 2009, 2nd edition, 647 – 727.
[2.] Alexander, JrC., (2010). differential diagnosis in
surgical pathology of the lung and pleura, 2nd edition, 217 – 280.
[3.] Barnard, R., & AM, J., (2008) Rapid Molecular
Screening for multidrug- resistant tuberculosis in a high volume public health
laboratory, 177:787 – 792, South Africa.
[4.] Brooks, GF et al (1995): Jawetz, Melnick, &
Adelberg’s Medical Microbiology, 21ST International Edition, Prentice Hall
International Limited, London, UK, 134 – 144.
[5.] Caviedes, L., & LEE, TS., (2000). Rapid efficient
detection and drug susceptibility testing of Mycobacterium Tuberculosis in
sputum by microscopic observation of broth cultures; 38; 1203 – 1208,
tuberculosis working groups in Peru.
[6.] Centres for Disease Control and Prevention, MMWR
Recomm, 2002, sexually transmitted diseases treatment guidelines, 51(RR – 6): 1
– 78.
[7.] Gavino, M., &Wang, E., (2007)., a comparison of a
new rapid real – time polymerase chain reaction system to traditional culture
in determining group B streptococcus colonization.
[8.] Goldman’s Cecil medicine,(2012), non tuberculous
mycobacteria, volume 2, pages 1948 – 1950, 24th edition, Steven M. Holland.
[9.] Margret, S., et al, 2009, nonsquamous pathological
diseases of the hypophyarnx, larynx and trachea, chapter 5, 2nd edition and
pages 309 – 411.
[10.] Mitnick, CD., & S.S. (2008)., comprehensive
treatment of extensively drug – resistant tuberculosis. N. Engl. J. MED,
359; 563 – 54.
[11.] Nakajima et al 2010, identification of mycobacterium
tuberculosis clinical isolates in Bangladesh by species distinguishable
multiplex PCR, 10:118, http:/ www.Biomedcentral.com/1471 – 2334/10/118.
[12.] Rutala, W.A., & D.J. (2002)., draft guideline for
disinfection and sterilization in healthcare facilities, centres for disease
control and prevention, Atlanta.
[13.] Steven, L., (2014). microbiology of waterborne
diseases, second edition, chapter nine pages 177 – 207. Mycobacterium.
[14.] Temple, I.. Ayakaka, S., & Ogwang, (2008). rate
and amplification of drug resistance among previously treated patients with
tuberculosis in Kampala, Uganda; 47:1/26 – 1134.
[15.] Basil, MVH., (2004). Rapid detection of rifampin
resistance in mycobacterium tuberculosis isolates from India and Mexico by a
molecular bacon assay. 42:5512 – 5516.
[16.] World Health Organization, WHO/IUATLD global project,
2008 Anti – tuberculosis drug resistance in the world. Publication number
WHO/HTM/TB 2008:394, WHO, Geneva and Switzerland.