Abstract:

The present study was designed to investigate the local anesthetic activity of EMLA cream on intact skin of hind limbs of New Zealand white rabbits by employing a novel method “Pedaling reflex in rabbits”. Five different doses of drug ranging from 0.5 to 2.5 mg was selected and applied to the ventral surface of the hind feet of both limbs. Uniform pressure was applied on anesthetized patches with help of a blunted probe. Six pricks were applied at different points on anesthetized patches and loss of pedaling reflex (leg retraction) followed by application of stimulus was considered as sign of anesthesia. A significant reduction in onset of anesthesia and a dose dependent increase in duration of anesthesia were recorded in the present study. The onset and duration of action for local anesthetic EMLA cream depends on dosage and contact time of drug on intact skin.

Keywords: White rabbit, EMLA cream

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